Analysis of 12 X-chromosomal markers in the population of central Croatia |
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| Institution: | 1. University Department for Forensic Sciences, University of Split, Split, Croatia;2. Ruđer Bošković Institute, Zagreb, Croatia;3. Forensic Science Centre “Ivan Vučetić”, Zagreb, Croatia;4. Forensic Science Office, University of Zagreb, Zagreb, Croatia;5. Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia;1. Department of Family Genetics, Norwegian Institute of Public Health, P.O. Box 4040 Nydalen, NO-0403 Oslo, Norway;2. Department for Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway;3. Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, SE-587 58 Linköping, Sweden;4. Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden;1. Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCI-UdeG), Ocotlán, Jalisco, Mexico;2. Laboratorio de Genética Forense, Instituto de Criminalística y Servicios Periciales, Procuraduría General de Justicia, Nuevo León, Mexico;3. Laboratorio de Genética Forense, Servicios Periciales de la Procuraduría General de Justicia del Estado de Baja California, BC, Mexico;4. Laboratorio de Genética del Instituto de Ciencias Forenses, Poder Judicial del Distrito Federal, México DF, Mexico;5. Departamento de Zoología y Antropología Física, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain;6. Institut Universitari d’Investigació en Ciències de la Salut (IUNICS) i Laboratori de Genètica, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122 Palma de Mallorca, Illes Balears, Spain;1. DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman, Philippines;2. Philippine Genome Center, University of the Philippines, Philippines;3. Balik Scientist, Philippine Council for Industry, Energy and Emerging Technology Research and Development – Department of Science and Technology, Philippines;1. Institute of Pathology and Molecular Immunology from University of Porto (IPATIMUP), Portugal;2. Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal;3. CMUP, Centro de Matemática da Universidade do Porto, Portugal;4. Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark;5. Laboratório de Diagnóstico por DNA (LDD), Universidade do Estado do Rio de Janeiro, Brazil;6. Centro de Genética Forense, Poder Judicial de Córdoba, Argentina;7. Laboratorio Regional de Investigación Forense, Tribunal Superior de Justicia de Santa Cruz, Argentina;8. UNESP-Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Laboratório de Investigação de Paternidade-NAC, São Paulo, Brazil;9. Instituto Nacional de Medicina Legal e Ciências Forenses, I.P., Serviço de Genética e Biologia Forenses, Delegação do Centro, Portugal;10. Escuela de Medicina, Facultad de Ciencias de la Salud, Universidad de Las Américas (UDLA), Quito, Ecuador;11. Laboratorio de ADN de la Fiscalía General del Estado, Quito, Ecuador;12. Departamento de Biología, Servicio de Criminalística de la Guardia Civil, Spain;13. Laboratorio Regional de Genética Forense, Poder Judicial de Río Negro, Argentina;14. Instituto Nacional de Medicina Legal e Ciências Forenses, I.P., Serviço de Genética e Biologia Forenses, Delegação do Norte, Portugal;15. PRICAI-Fundación Favaloro, Buenos Aires, Argentina;p. Laboratorio de Análisis de ADN Facultad de Ciencias Médicas Universidad Nacional de Cuyo, Mendoza, Argentina;q. LabGenetics: Laboratorio de Genética Clínica S.L., Madrid, Spain;r. Genomic Engenharia Molecular Molecular, São Paulo, Brazil;s. Laboratorio de Genética Forense, Poder Judicial de la Provincia de La Pampa, Argentina;t. Laboratorio MANLAB, Área de Filiaciones, Buenos Aires, Argentina;1. Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, (UNAM), Ciudad Universitaria, México DF, Mexico;2. Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCiénega-UdeG), Ocotlán, Jalisco, Mexico;3. Laboratorio de Genética Forense, Instituto de Criminalística y Servicios Periciales, Procuraduría General de Justicia, Nuevo León, Mexico;1. Department of Medical and Surgical Sciences, DIMEC, Institute of Legal Medicine, University of Bologna, Italy;2. Department of Biological, Geological and Environmental Sciences, BIGEA, Laboratory of Molecular Anthropology, University of Bologna, Italy |
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| Abstract: | Investigator® Argus X-12 Kit is a commercially available set that allows simultaneous PCR amplification of 12 X-STR markers belonging to four linkage groups (LG). To assess the forensic efficiency of these markers for the population of central Croatia and consequent applicability in routine forensic casework, DNA from 200 blood samples of unrelated donors (100 female and 100 male) was amplified by Investigator® Argus X-12 Kit and analyzed by capillary electrophoresis. Statistical computations based on allele and haplotype frequencies for LG1 – LG4 were performed using Arlequin 3.5 software and on-line tool available at ChrX-STR.org. In female samples, all X-STR markers were in Hardy-Weinberg equilibrium (HWE). The most informative marker for central Croatia population was DXS10135 with polymorphism information content (PIC) 0.9296. The least polymorphic locus was DXS8378 (PIC = 0.6363). Power of discrimination (PD) varied from 0.6968 to 0.9336 in male and from 0.8476 to 0.9916 in female samples. Combined PD exceeded 0.999999999 in both men and women. In male samples, linkage disequilibrium (LD) test revealed significant association (P = 0.0000) of one marker pair in LG4 and two marker pairs in LG3. Portion of observed haplotypes in the number of possible haplotypes varied from 2.86% to 7.47% across all LGs. LG1 was the most informative with haplotype diversity (H) 0.9972. High PD of all analyzed markers exhibited for central Croatia population confirms suitability of Investigator® Argus X-12 for forensic pertinence. Moreover, results of this study will be included in establishing a national reference X-STR database based on 12 X-STR loci, which is necessary for the correct interpretation of the forensic casework results. |
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| Keywords: | X-STR Investigator® Argus X-12 Population study Forensics Central Croatia |
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