抗坏血酸(VC)对氯化锂诱导的腹腔巨噬细胞氧化损伤的保护作用

目的观察抗坏血酸(VC)对氯化锂诱导的腹腔巨噬细胞氧化损伤的保护作用。方法采用体外细胞培养法测定在VC(10、20、40、60、80、100μg//L)和氯化锂(50μg//L)作用下,测定体外VC和氯化锂对腹腔巨噬细胞的细胞抑制率、NO、H2O2及O2-指标。结果在各剂量VC和氯化锂作用下,巨噬细胞吞噬功能受到明显抑制,细胞抑制率分别为83.9%、87.5%、92.2%、96.9%,且呈现剂量-反应关系。巨噬细胞产生NO、H2O2及O2-的含量也受到明显抑制,呈现剂量-反应关系。结论 VC对巨噬细胞的吞噬功能,NO、H2O2及O2-等生物分子的产生均有明显促进作用,因此认为VC可影响巨噬细胞的非特异性防御功能。

The protection of Ascorbic acid(VC)to oxidative damage of abdominal macrophages inducted by LiCl

OBJECTIVE To observe the protection of ascorbic acid(VC)to the oxidative damage of abdominal macrophages inducted by LiCl.METHODS In vitro cell culture with the VC(10,20,40,60,80,100 μg/L)and lithium chloride(50μg/L),determined the inhibition rate of abdominal macrophage,NO,H2O2 and O2-indicators affected by VC and lithium chloride.RESULTS Under the action of various doses of VC and lithium chloride,the phagocytosis of abdominal macrophage was obviously inhibited,cell inhibition rates were 83.9%,87.5%,92.2% and 96.9%,and presented the relation of dose-response.The content of NO,H2O2 and O2-produced by macrophages was also obviously inhibited,and presented the relation of dose-response.CONCLUSION VC promotes the phagocytosis of macrophage,the production of NO,H2O2 and O2-and other biological molecules significantly,so we can think that VC can affect the nonspecific defense functions of macrophages.