五味子酚对氧自由基损伤小鼠脾淋巴细胞的保护作用

研究了五味子酚(Sal)对氧自由基损伤小鼠脾淋巴细胞的影响。体外实验结果表明,Sal在5×10^-6mol·L^-1时对Fe²⁺-VitC引起的脾淋巴细胞GSH含量降低有明显的抑制作用,且能阻抑Fe²⁺-Cys引起的MDA生成增加,改善细胞膜的流动性。用扫描电镜观察到Sal在5×10^-4mol·L^-1时可逆转Fe²⁺-VitC引起的脾淋巴细胞表面微绒毛皱折减少、细胞变形等病理改变。体内高氧分压应激损伤小鼠实验表明,igSal20mg·kg^-1×8d可逆转脾淋巴细胞SOD活性代偿性增高,并提高脾淋巴细胞内GSH含量。以上结果提示Sal对氧自由基损伤脾淋巴细胞有保护作用。

PROTECTIVE EFFECT OF SCHISANHENOL (SAL) ON OXYGEN FREE RADICAL INDUCED INJURY OF SPLEEN LYMPHOCYTES IN MICE

In the present paper the effects of Sal, an active component isolated from Kadsura Longipenduculata Finet et Gagnep, on oxygen free radical induced injury of murine spleen lymphocytes in vitro and in vivo were investigated. The results showed that Sal at 1 x 10(-4) mol.L-1 significantly inhibited the decrease of GSH content induced by Fe(2+)-Vit C and the increase of MDA formation induced by Fe(2+)-Cys in murine spleen lymphocytes. The decrease of membrane fluidity of lymphocytes under lipid peroxidation induced by Fe(2+)-Vit C was prevented by Sal. The damage of the lymphocyte surfaces induced by Fe(2+)-Vit C was also markedly reduced by Sal. Administration of Sal 20 mg.kg-1.d-1 for 8 d significantly increased the GSH content of the spleen lymphocytes in mice under high oxygen-stress. Meanwhile, the compensative increase of SOD activity in the spleen lymphocytes was reversed. The results suggest that Sal may protect murine spleen lymphocytes from the injury induced by oxygen free radicals.