鼻息肉基因芯片检测及其基因表达谱的研究

目的:运用基因芯片技术检测鼻息肉组织的基因表达谱,并通过分析基因的差异表达探讨相关基因在鼻息肉发病机制中的作用.方法:抽提6组鼻息肉和下鼻甲组织的总RNA,逆转录成cDNA,并分别用cy5和cy3标记制备成杂交探针,每组杂交探针与含有人类常见基因的BiostarH-40 s基因芯片杂交,用扫描仪扫描芯片荧光信号图像,并用软件对扫描图像进行数字化处理和分析.结果:鼻息肉组织基因表达谱中差异表达的基因共1887条,其中上调基因1099条,下调基因788条;6张芯片共同的差异基因6条,其中上调基因4条,下调基因2条.在差异表达的基因中,主要包括编码横跨膜4超家族蛋白、IFN-γ诱导蛋白IP-30前体、补体因子前体、胰岛素生长因子结合蛋白的基因.结论:检测鼻息肉基因表达谱中差异表达基因为研究鼻息肉的发病机制提供了分子生物学的线索和方法,鼻息肉为多基因调控,其中IFN-γ诱导蛋白、胰岛素样生长因子结合蛋白等基因可能在鼻息肉发病中起重要作用.

Research of gene chip detection and gene expression profile of nasal polyps

Objective:To investigate the gene expression profile by using gene chip technology and probe into the role of corresponding gene in the pathogenesis of nasal polyps by analysing the difference of the gene depression.Method:The total RNAs were respectively extracted from 6 pairs of inferior turbinates and nasal polyps,and then were reversely transcribed to cDNAs with incorporation of fluorescent dUTP as the hybridization probes.The mixed probes were then hybridized with the BiostarH-40 s gene chips,it was scanned by laser scanner and the acquired image was analyzed by software.Result:1 887 genes were differently expressed in gene profile of nasal polyps,among which 1 099 were upregulated and 788 were down-regulated.Six genes were found in all gene chips,among which 4 genes were upregulated and 2 were down-regulated.The 6 genes encoded the protein of the transmembrane 4 superfamily,highly similar to GAMMA-interferon-inducible protein IP-30 precursor,highly similar to complement factor I precursor and insulin-like growth factor binding protein 3(IGFBP3).Conclusion:Detecting the differently expressed genes will provide clues and theoretical foundation for the pathogenesis of nasal polyps.The nasal polyps is a polygenic disease and the genes of GAMMA-interferon-inducible protein,insulin-like growth factor binding protein may play an important role in its pathogenesis.