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Enzyme-linked immunosorbent assay for detection of antibodies toPseudomonas aeruginosa exoproteins |
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| Authors: | M. Granström B. Wretlind B. Markman O. R. Pavlovskis M. L. Vasil |
| Affiliation: | 1. Department of Bacteriology, National Bacteriological Laboratory, S-10521, Stockholm, Sweden 2. Department of Vaccine Production, National Bacteriological Laboratory, S-10521, Stockholm, Sweden 3. Department of Clinical Microbiology, Karolinska Hospital, S-10401, Stockholm, Sweden 4. Naval Medical Research Institute, 20814, Bethesda, Maryland, USA 5. Department of Microbiology and Immunology, University of Colorado Health Sciences Center, 80262, Denver, Colorado, USA |
| Abstract: | Enzyme-linked immunosorbent assays were developed with four purifiedPseudomonas aeruginosa extracellular proteins (exotoxin A, elastase, alkaline protease, and phospholipase C) to determine antibody levels in sera from healthy subjects and the serological response in patients colonized or infected withPseudomonas aeruginosa. Five of 39 burn patients with wounds colonized byPseudomonas aeruginosa had elevated antibody titers to alkaline protease. Response to the other antigens was found in only a few patients.Pseudomonas aeruginosa infections (septicemia, osteitis, pneumonia etc.) resulted in increased antibody levels to exotoxin A or phospholipase C in 15 of 22 patients. These findings suggest that repeated determinations of antibodies toPseudomonas aeruginosa exotoxin A and phospholipase C might be used to monitor therapy in certain patients with osteitis and other deepPseudomonas infections. |
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