N-乙酰-L-半胱氨酸对氰化钠急性中毒小鼠的解毒作用

目的：研究N-乙酰-L-半胱氨酸（NAC）对氰化钠（NaCN）的解毒作用。方法：观察并记录4种巯基化舍物腹腔注射保护后，NaCN急性中毒小鼠的行为变化及72h内惊厥和死亡鼠数；通过急性毒性实验测定NaCN组和NAC保护组急性小鼠LD50；观察并比较灌胃NaCN染毒1 min后，NAC联用硫代硫酸钠（Na2 S2 O3）组与亚硝酸钠（NaNO2）联用Na2 S2 O3组小鼠的行为变化及72h惊厥和死亡鼠数。结果：4种巯基化合物对NaCN急性中毒小鼠都有不同程度的保护作用，其中NAC效果最显著（P〈0．01）；经腹腔注射NAC保护后，能显著提高NaCN急性中毒小鼠LD50（P〈0．01）；小鼠腹腔注射NaCN染毒1 min后NAC联用Na2 S2 O3治疗，72h内惊厥数和死亡数显著下降（P〈0．01），与NaNO2联用Na2 S2 O3组无差异。结论：巯基化合物具有抗氰作用，且与其巯基相关，NAC对NaCN急性中毒有明显解毒作用。

Antidotal effect of N-acetylcysteine on acute poisoning with sodium cyanide

AIM: To study the antidotal effect of N-acetylcysteine(NAC) on acute poisoning with sodium cyanide(NaCN).METHODS:After intraperitoneal(ip) injected of four sulphydryl compounds respectively,the mice which were acutely poisoned by NaCN were observed of the behavioral change,convulsion number and mortality rate(within 72 h).The median lethal dose(LD_(50)) of acutely poisoned mice was detected in NaCN group and NAC protection group.1 min after mice were poisoned by NaCN,the mice were divided into three groups:the first was ip normal saline(NS),the second was ip NAC in combination with sodium thiosulfate(Na_2S_2O_3),the third was ip sodium nitrite(NaNO_2) in combination with Na_2S_2O_3.Then the behavioral change,convulsion number and mortality rate(within 72 h)were observed,recorded,and compared the differences between the mice groups.RESULT: All of the four sulphydryl compounds had protecting effect on acutely NaCN poisoned mice,among them NAC had most prominent effect(P<(0.01)).LD_(50) were raised significantly(P<(0.01)) in acutely NaCN poisoned mice pretreated with ip NAC.After NaCN poisoned for 1 min,in the group of treatment with ip NAC in combination with Na_2S_2O_3,convulsion number and mortality of mice(within 72 hours) were declined significantly(P<(0.01)).It was not significant different when compared with the group which were treated by ip NaNO_2 in combination with Na_2S_2O_3.CONCLUSION: Sulphydryl compounds antagonizing cyanide intoxication are related with Sulphydryl.All of the results indicate that NAC has the antidotal effects on acutely NaCN poisoned animals.