职业接触环氧丙烷工人Hb加合物及其遗传毒性分析

目的 研究长期接触低浓度环氧丙烷工人的遗传效应。方法 采用气相色谱法对环氧丙烷作业现场进行监测的同时，采集工人血样，采用高灵敏的N-alkyl Edman气质谱法对血红蛋白加合物(HP-缬氨酸)含量进行了测定，同时对姐妹染色单体互换(SCE)进行检测。结果 环氧丙烷作业工人时间加权平均浓度(TWA)为2．6mg/m^3和6．9mg／m^3。环氧丙烷接触组工人的HP-缬氨酸水平明显高于对照组，差异有极显著性(P=0．0018)。接触组中的每个细胞SCE数明显高于对照组，差异有显著性(P=0．011)。血红蛋白加合物与姐妹染色单体互换的相关系数r为0．762．并且差异有极显著性(P=0．0014)。结论 低浓度环氧丙烷对职业人群具有细胞遗传学毒性。

Anallysis on hemoglobin adducts and its cytogenetic effect in workers exposed to propylene oxide

Objective To study the cytogenetic effect of workers exposed to low level propylene oxide in longer time.Methods Gas chromotography(GC) was used to detect the propylene oxide level in workshop air,the worker's blood samples was also collected.Hemoglobin adducts(HP valine)were measured by the N alkyl GC/MS method,the sister chromatid exchange(SCE)frequency was also analyzed.All data were analyzed by SPSS 8 0 statistical software.Results The TWA of two propylene oxide exposed posts was 2 6 and 6 9 mg/m 3,respectively.The level of HP valine of PO workers was significantly higher(P=0 001 8).The SCE frequency was also significantly higher in exposed workers compared to the controls(P=0 011).There was also a obvious correlation between HP valine levels and SCE frequency(r=0 762,P=0 001 4).Conclusion Low level propylene oxide has cytogenetic effect to the workers.