嗅鞘细胞对原代培养神经干细胞增殖、分化的影响

目的 观察嗅鞘细胞(OECs)对神经干细胞(NSCs)增殖、分化的影响.方法 新生大鼠脑OECs和NSCs原代培养,采用免疫荧光及免疫细胞化学方法鉴定相关细胞.取原代OECs分为2组,实验组去除培养孔的间隔,使OECs和NSCs共用一培养液体系;对照组不破坏培养孔的间隔,单独培养NSCs,其余同实验组.观察2组细胞增殖、分化情况.结果 原代培养的OECs表达神经生长因子受体(P75NGFR);原代培养的神经球表达巢蛋白(nestin),神经球分化的细胞表达神经丝200(NF200)和胶质纤维酸性蛋白(GFAP).增殖实验中,实验组NSCs数量较对照组明显增多,差异有统计学意义(P<0.05).诱导分化实验中,实验组4d、7d时NF200阳性细胞率较对照组明显升高,差异有统计学意义(P<0.05),说明2种细胞共液培养时,OECs提高了NSCs向NF200阳性细胞分化率.结论 OECs可促进NSCs增殖,并提高了NSCs向神经元分化的效率.

Effect of olfactory ensheathing cells on proliferation and differentiation of neural stem cells in primary culture

Objective To explore the effect of olfactory ensheathing cells (OECs) on the proliferation and differentiation of neural stem cells (NSCs). Methods The cells from the embryonic rat brain were primarily cultured and identified by immunofluorescence and immunocytochemistry. NSCs in the experimental group shared medium with OECs were cultured and induced to differentiate. Simultaneously, NSCs in the control group were cultured alone. The effect of OECs on the proliferation and differentiation of NSCs was observed by immunocytochemistry. Results Nerve growth factor receptor (P75NGFR) was observed in the primarily cultured OECs; nestin was expressed in the primarily cultured neurosphere and the cells differentiated from the neurosphere expressed neurofilament 200 (NF200) and glial fibrillary acidic protein (GFAP). Compared with that in the control group, the number of NSCs in the experimental group was significantly increased (P<0.05). On the 4~(th) and 7~(th) day of differentiation, the percentage of NF200-positive cell was higher in the experimental group than that in the control group (P<0.05), indicating that the appearance of OECs increased the differentiation of NSCs into NF200-positive cells. Conclusion OECs can promote the proliferation of NSCs and induce the differentiation of NSCs into neurons.