上游刺激因子1在小鼠牙齿发育过程中表达的原位杂交研究

目的：检测小鼠牙齿发育中上游刺激因子1mRNA的表达及时空分布模式。方法：制备E13,16,19,P1,5,8,11,21d小鼠第一磨牙石蜡切片,用我们前期实验制备的USF1cDNA探针,原位杂交方法检测USF1mRNA的表达和分布。结果：原位杂交染色从钟状晚期小鼠牙胚中开始检测到阳性信号,持续至P11d,阳性信号主要定位于成牙本质细胞与成釉细胞;而蕾状期,帽状期,钟状期牙胚中未检测到阳性信号,并且在牙齿萌出后（P21d）,牙齿中USF1mRNA阳性信号再次消失。结论：牙胚中有USF1mRNA表达,主要位于分泌期的成牙本质细胞和成釉细胞,表达模式具有显著的时空特异性。

In situ hybridization research of USF1 mRNA expression in mouse tooth development

AIM:To detect the temporo-spatial expression of USF1 mRNA during mouse tooth development.METHODS:Paraffin sections of first molar tooth germ in E13,16,19,P1,5,8,11,21d mouse were prepared respectively and in situ hybridization was carried out with the specific USF1 cDNA probe prepared in our previous researches.RESULTS:Positive staining for USF1 mRNA in mouse tooth germ began from P1d evidently,and extended to P11d,which was mainly localized in the secreting ameloblasts and odontoblasts,but no staining in bud,cap and early bell stages of tooth germ.Moreover,on P21d after tooth eruption,USF1 mRNA became negative again.CONCLUSION:USF1 mRNA is expressed in tooth germ,which mainly localizes in the secreting ameloblasts and odontoblasts,and its expression was quite dynamic during mouse tooth development.