Interaction of the apolipoprotein E receptors low density lipoprotein receptor-related protein and sorLA/LR11 |
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| Authors: | R. Spoelgen K.W. Adams M. Koker A.V. Thomas O.M. Andersen P.J. Hallett K.K. Bercury D.F. Joyner M. Deng W.H. Stoothoff D.K. Strickland T.E. Willnow B.T. Hyman |
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| Affiliation: | 1. Department of Neurology/Alzheimer Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA;2. Max Delbrück-Center for Molecular Medicine, Robert-Roessle-Strasse 10, D-13125 Berlin, Germany;3. Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, 800 West Baltimore Street, Baltimore, MD 21201, USA;4. Present address: SYGNIS Pharma, 69120 Heidelberg, Germany;5. Present address: Department of Neurology, University of Cologne, 50931 Cologne, Germany;6. The MIND Center, Department of Medical Biochemistry, Aarhus University, 8000 Aarhus, Denmark;g Center for Neurogeneration Research, McLean Hospital/Harvard Medical School, Belmont, MA 02478, USA |
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| Abstract: | In this study, we examined protein–protein interactions between two neuronal receptors, low density lipoprotein receptor-related protein (LRP) and sorLA/LR11, and found that these receptors interact, as indicated by three independent lines of evidence: co-immunoprecipitation experiments on mouse brain extracts and mouse neuronal cells, surface plasmon resonance analysis with purified human LRP and sorLA, and fluorescence lifetime imaging microscopy (FLIM) on rat primary cortical neurons. Immunocytochemistry experiments revealed widespread co-localization of LRP and sorLA within perinuclear compartments of rat primary neurons, while FLIM analysis showed that LRP-sorLA interactions take place within a subset of these compartments. |
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| Keywords: | LRP sorLA LR11 LDL receptor |
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