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| 苦荞中肌醇含量的测量方法研究 | |
| 引用本文： | 宾婕,鲁卫东,王韵,陈克嶙,周劲,郭亚东. 苦荞中肌醇含量的测量方法研究[J]. 昆明医学院学报, 2011, 32(11): 40-42 |
| 作者姓名： | 宾婕 鲁卫东 王韵 陈克嶙 周劲 郭亚东 |
| 作者单位： | 1. 昆明医学院药学院,云南昆明,650500 2. 云南农业大学动物科技学院,云南昆明,650201 3. 云南侨通包装印刷有限公司,云南昆明,657000 |
| 基金项目： | 云南侨通包装印刷有限公司资助项目(kx140564); 昆明医学院药学院大学生创新基金资助项目(DXSCX-2010-04);昆明医学院研究生创新基金项目(2011J11) |
| 摘 要： | 目的为得到一种简单、快速、准确测定苦荞中肌醇的方法.方法采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法进行测定,色谱柱为美国Phenomenex公司提供的Luna C18柱（4.6 mm×150 mm,3μm）,流动相为甲醇-水（含0.1%的冰醋酸）,流速：1 mL/min.漂移管温度50℃,载气压力40 psi.结果肌醇进样量在0.0268～0.1340μg范围内（r=0.9997）线性关系良好,平均加样回收率为99.7%（相对标准偏差RSD=3.49%）.结论该方法简便可靠,可用于苦荞中肌醇含量的质量控制. |
| 关 键 词： | 苦荞 肌醇 高效液相色谱蒸发光检测 含量测定 |
Measuring Methods of Inositol Contetin Buckwheat |
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| BIN Jie,LU Wei-dong,WANG Yun,CHEN Ke-lin,ZHOU Jin,GUO Ya-dong. Measuring Methods of Inositol Contetin Buckwheat[J]. Journal of Kunming Medical College, 2011, 32(11): 40-42 | |
| Authors: | BIN Jie LU Wei-dong WANG Yun CHEN Ke-lin ZHOU Jin GUO Ya-dong |
| Affiliation: | BIN Jie1),LU Wei-dong1),WANG Yun1),CHEN Ke-lin2),ZHOU Jin3),GUO Ya-dong1)(1)School of Pharmacy,Kunming Medical University,Kunming Yunnan 650500,2)College of Animal Science and Technology,Yunnan Agricultural University,Kunming Yunnan 650201,3)Yunnan Overseas Package Printing Co.,Ltd,Kunming Yunnan 657000,China) |
| Abstract: | Objective To explore a simple,rapid and accurate method for detecting inositol content in buckwheat.Methods HPLC-ELSD method was used to detect the content of inositol in buckwheat.A Phenomenex Luna-C18 column（4.6 mm×150 mm,3 μm）was used for separation.Methylalcohol-Water（containing 0.1% glacial acetic acid）was used as mobile phase at a flow rate of 1 mL/min.The temperature of drift tube was 50 ℃,and the gas pressure was 40 psi.Results Under the above described chromatographic conditions,the linear ranges of inositol was 0.0268～0.1340 μg（r=0.9997）,and the mean recovery rate was 99.7%（RSD=3.49%）.Conclusion The assay method is simple and accurate for the quality contro1 of inositol in buckwheat. |
| Keywords: | Buckwheat Inositol HPLC-ELSD Content determination |
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