| HPLC-ELSD法测定咳喘宁中桔梗皂苷D的含量 |
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| 引用本文： | 郑琰,张秀丽,崔岩.HPLC-ELSD法测定咳喘宁中桔梗皂苷D的含量[J].中国当代医药,2014,21(14):11-13. |
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| 作者姓名： | 郑琰 张秀丽 崔岩 |
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| 作者单位： | 郑琰(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000)；张秀丽(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000)；崔岩(辽宁省朝阳市食品药品检验所,辽宁朝阳,122000)； |
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| 摘 要： | 目的 建立高效液相色谱法测定咳喘宁中桔梗皂苷D的方法.方法 采用以蒸发光散射检测器的高效液相色谱法,色谱柱为Kromasil C18（4.6 mm×250.0 mm,5μm）,流动相为乙腈∶水（28∶72）,流速为0.9 ml/min,ELSD漂移管温度为70℃,气体流速为3.0 SLPM/min.结果 桔梗皂苷D进样量在0.513～10.260 μg范围内呈良好线性关系（r=0.9996）,平均回收率98.44％,RSD=0.88％ （n=6）.结论 该方法准确,可靠,重复性好,灵敏度高,可用于咳喘宁的质量控制标准.
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| 关 键 词： | 咳喘宁 桔梗皂苷D 高效液相色谱法-蒸发光散射检测器 |
Determination of platycodin D in Kechuanning by HPLC-ELSD method |
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| Institution: | ZHENG Yan ZHANG Xiu-li CUI Yah (Institute for Food and Drug Control of Chaoyang City in Liaoning Province,Chaoyang 122000,China) |
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| Abstract: | Objective To establish a method for determination the content of platycodin D in Kechuanning. Methods High performance liquid chromatography with evaporative light scattering detector （HPLC-ELSD）method was used,the column was Kromasil C1s（4.6 mm×250.0 mm,5 μm）,mobile phase was acetonitrile ： water（28：72）.The flow rate was 0.9 ml/min, the ELSD drift tube temperature was 70℃ and the gas flow rate was 3.0 SLPM/min. Results The linear range of Platy- codin D was 0.513-10.260 μg（r=0.9996）.The average recovery was 98.44%,RSD=O.88%（n=6）. Conclusion The method is accurate,reliable,good reproducibility,high sensitivity and can be used for the quality control of Kechuanning standard. |
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| Keywords: | Kechuanning Platycodin D HPLC-ELSD |
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