Application of spin-trapping study to rat ischemic brain homogenate incubated with NADPH and Fe-EDTA |
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| Authors: | T Tominaga S Imaizumi T Yoshimoto J Suzuki Y Fujita |
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| Affiliation: | 1. Korea Food Research Institute, Jeollabukdo 55365, South Korea;2. Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, South Korea;3. Institution of Genetic Science, Yonsei University College of Medicine, Seodaemun-gu, Seoul 03722, Republic of Korea;4. Division of Cardiology, Department of Medicine, Johns Hopkins University, MD, 21205, Baltimore, USA;1. Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan 450046, China;2. Henan University of Chinese Medicine, Zhengzhou, Henan, China;3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;1. Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, PR China;2. Department of Biochemistry and Molecular Biology, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, PR China;3. College of Agronomy, Shandong Agriculture University, Tai''an, Shandong 271018, PR China |
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| Abstract: | ![]()
A spin-trapping technique was applied to the detection of free radicals generated in nicotinamide-adenine denucleotide phosphate (NADPH)-dependent lipid peroxidation of rat ischemic brain homogenate. The spin adduct of phenyl-t-butylnitrone (AN = 16.2-16.5 g, AH beta = 3.6-3.8 g) was observed, and it was thought to be derived from NADPH-dependent lipid peroxidation from its oxygen and NADPH dependency. Its intensity was increased in the recirculated condition following ischemic insult, indicating the susceptibility of brain tissue to lipid peroxidation in such a condition. |
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