| 硝酸纤维素膜在细胞培养和染色中的应用 |
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| 引用本文： | 张泓,王亚东,赖卓胜. 硝酸纤维素膜在细胞培养和染色中的应用[J]. 南方医科大学学报, 2003, 23(1): 89-90 |
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| 作者姓名： | 张泓 王亚东 赖卓胜 |
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| 作者单位： | 第一军医大学南方医院外科实验室,广东,广州,510515;第一军医大学南方医院全军消化病研究所,广东,广州,510515 |
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| 摘 要： | 目的提供一种新的用于细胞培养和病理技术的支持介质。方法应用硝酸纤维素膜培养细胞和病理染色并与常规方法培养细胞的比较。结果细胞生长良好,形态正常,染色后结构清晰,与常规玻璃培养的细胞无明显差异。结论硝酸纤维素膜具有良好的透光性及化学稳定性,无细胞毒性作用,同时对细胞有较好的亲合性,可以替代玻片,较玻片具有广阔的应用前景。
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| 关 键 词： | 硝酸纤维素膜 细胞培养 病理染色 |
| 文章编号： | 1000-2588(2003)01-0089-02 |
| 修稿时间： | 2002-07-25 |
Application of nitrocellulose membrane in cell culture and pathological techniques |
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| ZHANG Hong ,WANG Ya-dong ,LAI Zhuo-sheng Surgical Laboratory. Application of nitrocellulose membrane in cell culture and pathological techniques[J]. Journal of Southern Medical University, 2003, 23(1): 89-90 |
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| Authors: | ZHANG Hong WANG Ya-dong LAI Zhuo-sheng Surgical Laboratory |
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| Affiliation: | ZHANG Hong 1,WANG Ya-dong 2,LAI Zhuo-sheng 21 Surgical Laboratory,2 Institute of Digestive Disease of PLA,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To provide a new supporting medium for carrying out cell culture and histological staining. Methods LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control. Results The cells cultured on nitrocellulose membrane showed no visible difference from those cultured on coverglasses in terms of cell growth, morphology, and the structure displayed after staining. No toxic effect was observed due to the application of nitrocellulose membrane. Conclusions Nitrocellulose membrane after treatment has such merit as good lucidity and stability without toxicity on the cells. Having also good affinity with the cells, nitrocellulose membrane can offer an promising alternative for glass as cell culture medium. |
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| Keywords: | nitrocellulose membrane cell culture pathology |
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