不同途径接种乙脑毒prME蛋白编码基因DNA疫苗所致BALB/c鼠的细胞免疫

目的:研究经不同途径接种乙脑病毒prME蛋白编码基因DNA疫苗所诱导的细胞免疫。方法:将乙脑病毒prME蛋白编码基因DNA疫苗(命名为pJME)分别以肌注、滴鼻两种免疫方式免疫BALB/c鼠,流式细胞仪检测脾脏CD4 /CD8 T淋巴细胞亚群,乳酸脱氢酶释放实验测定脾脏特异性细胞毒性T淋巴细胞(CTL)活性。结果:pJME肌注组与滴鼻组免疫鼠CD4 T淋巴细胞百分比(30.91 0.72)%、(27.89 0.42)%,均高于灭活疫苗与载体肌注组(23.73 0.17)%、(23.78 0.21)%(P<0.05),肌注组显著高于滴鼻组(P<0.05);而CD8 T淋巴细胞百分比(14.62 0.25)%、(13.73 0.50)%,也均高于灭活疫苗和载体肌注组(10.80 0.39)%、(11.97 0.06)%(P<0.05),肌注组与滴鼻组无显著差异(P>0.05);两组有明显CTL效应(pJME肌注组29.1%,pJME滴鼻组17.1%),明显高于载体肌注组6.0%,pJME肌注组明显优于滴鼻组。结论:pJME以两种途径免疫鼠时可诱导明显细胞免疫反应。

Cellular immunity of BALB/c mice induced by DNA vaccine encoding prME protein derived from Japanese encephalitis virus in the routes of different inoculation

Objective:To study cellular immunity induced by DNA vaccine encoding prME protein derived from Japanese encephalitis virus in the routes of different inoculation.Methods:BALB/c mice were vaccinated with DNA vaccine,named pJME,encoding Japanese Encephalitis virus prME protein via intramuscular and intranasal injection respectively.CD4 /CD8 T lymphocyte subsets of mouse spleen cells were assayed with flow cytometry technique,and CTL activity was detected with lactic dehydrogenase release test method.Results:Both of CD4 T lymphocyte percentage [intramuscular:(30.91 0.72)%,intranasal:(27.89 0.42)%] produced by pJME via intramuscular and intranasal injection respectively were higher than that by inactivated vaccine and vector.[inactivated vaccine:(23.73 0.17)%,vector:(23.78 0.21)%](P < 0.05),however,pJME group via intramuscular injection was still higher than that via intranasal injection(P < 0.05).Both of CD8 T lymphocyte percentage [intramuscular:(14.62 0.25)%,intranasal:(13.73 0.50)%]produced pJME via intramuscular and intranasal injection respectively were also higher than that by inactivated vaccine and vector[inactivated vaccine:(10.80 0.39)%,vector:(11.97 0.06)%](P < 0.05),but there was no significant difference(P > 0.05) between pJME group via intramuscular injection and that via intranasal injection.Both of above groups could promote CTL activity(pJME intramuscular:29.1%,pJME intranasal:17.1%).pJME group via intramuscular injection was higher than that via intranasal injection.Conclusion:pJME via intramuscular and intranasal injection respectively can induce obvious cellular immunity.