| 出血性大肠杆菌单克隆抗体的制备与鉴定 |
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| 引用本文： | 赵志晶,刘秀梅.出血性大肠杆菌单克隆抗体的制备与鉴定[J].卫生研究,2002,31(2):108-111. |
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| 作者姓名： | 赵志晶 刘秀梅 |
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| 作者单位： | 中国预防医学科学院营养与食品卫生研究所,北京,100050 |
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| 基金项目： | 国家科技部科技工作专项经费资助 |
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| 摘 要： | 以肠出血性大肠埃希氏菌 (EnterohaemorrhagicE .coli,EHEC)O15 7∶H7免疫Balb c小鼠 ,取其脾细胞与小鼠骨髓瘤细胞Sp2 0融合、筛选 ,经 3次亚克隆建立了一个稳定分泌抗大肠杆菌O15 7单克隆抗体的杂交瘤细胞株 ,命名为 3A5。分泌抗体属IgM亚型 ,腹水的抗体效价达 1∶1× 10 6 。以辛酸 饱和硫酸铵法纯化后抗体的工作浓度为 1∶2× 10 4 ,检出限为 10 5～ 10 6 cfu ml。该抗体对大肠杆菌O15 7有较高特异性 ,同时抗出血性大肠杆菌O113∶H2 1抗原
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| 关 键 词： | 单克隆抗体 EHEC 大肠杆菌O157：H7 制备 鉴定 |
| 文章编号： | 1000-8020(2002)02-0108-04 |
| 修稿时间： | 2001年5月7日 |
Production and characterization of monoclonal antibody against the O antigen of Enterohemorrhagic Escherichia coli |
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| Zhao Zhijing,Liu Xiumei Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine,Beijing ,China.Production and characterization of monoclonal antibody against the O antigen of Enterohemorrhagic Escherichia coli[J].Journal of Hygiene Research,2002,31(2):108-111. |
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| Authors: | Zhao Zhijing Liu Xiumei Institute of Nutrition and Food Hygiene Chinese Academy of Preventive Medicine Beijing China |
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| Institution: | Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China. |
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| Abstract: | Spleen cells collected from Balb/c mice immunized with the somatic antigen of Enterohaemorrhagic E. coli O157:H7 are fused with murine Sp2/0 myeloma cells. A hybridoma cell line specific to E. coli O157 is established after screening and sub-cloning and is designated as 3A5. It belongs to a subtype of IgM and the ascetic titer of the antibody is 1:1 x 10(6). The detection limit is 10(5)-10(6) cfu/ml. This monoclonal antibody(McAb) also has activity with Enterohaemorrhagic E. coli O113:H21. |
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| Keywords: | EHEC |
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