急性阑尾炎患者脓液培养病原菌分布及耐药性分析

目的探讨引起急性阑尾炎患者感染的病原菌分布及耐药情况,为临床合理使用抗菌药物提供科学依据。方法标本经分离培养,用细菌鉴定仪对病原菌进行鉴定和药敏试验,用WHONET5.4软件对病原菌的药敏结果进行统计分析。结果共检出病原菌300株,其中革兰阴性杆菌218株(72.7%),革兰阳性菌77株(25.7%)。以大肠埃希菌检出率最高153株(51.0%),其次为金黄色葡萄球菌33株(11.0%)。产超广谱β-内酰胺酶(ESBLs)的大肠埃希菌和肺炎克雷伯菌的检出率52.9%和25.0%,耐甲氧西林金黄色葡萄球菌检出率为22.1%。药敏结果显示,病原菌对抗菌药物存在不同程度耐药性。结论急性阑尾炎患者感染主要致病菌为大肠埃希菌,不同病原菌对抗菌药物的敏感性不同,对脓液进行细菌培养和耐药性分析对临床合理选药非常重要。

Distribution and drug-resistance of pathogenic bacteria isolated from pus culture in patients with acute appendicitis

Objective To investigate： the distribution and the drug resistance of pathogenic bacteria in acute appendicitis, in order to provide scientific basis for clinic selection of antibiotics. Methods The strains of pathogenic bacterium were identified and antibiotics susceptibility tests were performed by the baciceria identifier. The data was analyzed by using WHONETS. 4 software. ResUlts A total of 300 strains of pathogenic bacteria were isolated from clinical samples. Gram negative bacilli had 218 strains, accounted for 72.7% and Gram positives had 77 strains, accounted for 25. 7%. E. coil （51. 0%） and S. aureus （11. 0%） were the main microorganism. 52. 9% E. coli and 25.0% Klebsiella pneumoniae produced ESBLS. The detection rate of MRSA was 22. 1%. The results of antibiotic susceptibility tests showed that the bacteria had different drug resistance. Conclusion The most common bacteria in acute appendicitis is E. coli and the sensitivity of different pathogens is different to antimicrobial agents. It is important to monitor and analyze the strains isolated from pus culture which will help the clinicians select antimicrobial drug reasonably.