凝聚胺法与微柱凝聚法检测不规则抗体的对比分析

目的探讨凝聚胺法与微柱凝聚法在不规则抗体筛选中的价值对比。方法对2010～2011年江门市新会区人民医院6 720例预约输血患者,用凝聚胺法与微柱凝聚法分别进行不规则抗体筛查,阳性结果再用谱细胞通过抗人球蛋白方法进行特异性检测。结果用微柱凝聚法筛查出不规则抗体阳性28例,阳性率为0.42%,而凝聚胺检测阳性26例,阳性率仅为0.39%,两种方法比较差异无统计学意义（P〉0.05）。其中Rh系统占总不规则抗体比例为57.1%,分别为抗-D 5例,抗-E 7例,抗-C 2例,抗-Ec 2例。结论凝聚胺法与微柱凝聚法都适用于临床筛查不规则抗体,微柱凝聚法比凝聚胺法更有利于大规模自动化筛查,对于有条件的大医院更适合临床输血前的应用和相关疾病的诊断,而凝聚胺法在基层或者临床急诊上也可合理开展。

The comparative analysis between microcolunm gel test and polybrene test in irregular antibody detection

Objective To explore the comparison of values of microcolunm gel test and polybrene test in irregular antibody detection.Methods From 2010 to 2011,6 720 cases with blood transfusion were test by microcolumn gel and polybrene for detecting irregular antibody,and then positive results were tested about antibody′s specificity by anti-human globulin test.Results 28 irregular antibody positive cases were detected by microcolumn gel test and positive rate was 0.42%.Only 26 cases were detected by polybrene test,and positive rate was 0.39%,there was no statistically significant difference between two methods（P0.05）.The positive rate of Rh blood group system antibody was 57.1% of irregular antibody,5 cases of them were Rh（D）,7 cases were Rh（E）,2 cases were Rh（C）,and 2 cases were Rh（Ec）.Conclusion Microcolunm gel test and polybrene test can be suitable to use in the clinical screening for irregular antibodies.Microcolunm gel is more conducive than polybrene for large-scale automated screening,and is more conducivefor clinical transfusion applications and disease diagnosis for large hospitals,and then polybrene test may be reasonable to carry out on the primary hospital or clinical emergency.