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Blinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri
Authors:Liao Robert S  Storch Gregory A  Buller Richard S  Orscheln Rachel C  Mardis Elaine R  Armstrong Jon R  Dunne W Michael
Affiliation:Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Abstract:We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II.
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