姜黄素对口腔舌癌细胞增殖与转移能力影响的实验研究

目的 探讨姜黄素对口腔舌癌细胞增殖与转移能力的作用及其作用机制.方法分别以0、5、10、20、30、60、100μmol·L-1姜黄素处理人舌癌细胞SCC-4 24 h,采用MTT法、侵袭实验、流式细胞仪、免疫荧光显微镜分别检测姜黄素对口腔舌癌细胞增殖与转移能力的影响.然后,采用cDNA微陈列芯片技术筛选和实时定鼍RT...

Anti-proliferative and anti-metastatic effects of curcumin on oral cancer cells

Objective The purpose of this article is to examine the effect of curcumin on the proliferation and metastasis of human tongue squamous cell carcinoma and analyze its mechanism. Methods SCC-4 were treated with curcumin of 0, 5, 10, 20, 30, 60, 100 μmol·L-1 in 24 h. MTT assay, Matrigel invasion assay, flow cytometry and fluorescence microscopy were used to examine the effect of curcumin on the growth and metastasis of SCC-4. cDNA microarray and RT-PCR were employed to analyze the  expression of genes treated by curcumin. Results The results showed that curcumin could concentration-dependently inhibit SCC-4 cell proliferation at the concentration range from 20 to 100 μmol·L-1. Furthermore, Matrigel invasion assay indicated that curcumin can reduce SCC-4 cell invasion under the dosage of 20, 30, 60 μmol·L-1. Flow cytometry also showed that curcumin can influence the distribution of cell cycle of SCC-4 cell with the dosage of 20, 30, 60 μmol·L-1. And the dosage of 30 μmol·L-1 curcumin could lead to the recruitment of alpha-tubulin. cDNA microarray showed that 87 genes were activated and 198 genes were inhibited with the effect of curcumin. These results were validated by the real time quantitative RT-PCR. Conclusion According to the results, it suggests that curcumin has the potential as the leading compound for anti-cancer proliferation and invasion in oral cancer treatment, and cdc27, EGFR substrate 15, PPAR-alpha and H2A histone may play an important role among this multiple anticancer-targeting ability.