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| LC-MS/MS法测定血浆中姜黄素的血药浓度 | |
| 引用本文： | 罗文汇,孙冬梅,高华宏,谭志灿. LC-MS/MS法测定血浆中姜黄素的血药浓度[J]. 中国药事, 2012, 26(11): 1246-1248 |
| 作者姓名： | 罗文汇 孙冬梅 高华宏 谭志灿 |
| 作者单位： | 1. 广东省中医研究所,广州,510095 2. 海南省三亚市中医院;广州中医药大学 3. 广东省中医研究所,广州 510095;广州中医药大学 |
| 摘 要： | 目的建立测定姜黄素血药浓度的方法。方法采用高效液相色谱-质谱联用法对姜黄素的血药浓度进行测定,采用固定相为Hypersil GOLD C18柱,以甲醇-水为流动相,进行梯度洗脱,流速为0.2mL.min-1,柱温为35℃;质谱采用高温电喷雾离子源,以选择反应监测模式进行定量分析,血浆样品用乙腈直接沉淀处理。结果姜黄素在2.5～187.5ng.mL-1范围内呈良好的线性关系。日内、日间RSD均小于15%（n=5）。结论本方法操作简单、灵敏度高、分析时间短,适用于姜黄素血药浓度监测及药代动力学研究。 |
| 关 键 词： | 液相色谱串联质谱 姜黄素 血药浓度 |
Determination of Curcumin in Plasma by LC-MS/MS |
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| Luo Wenhui , Sun Dongmei , Gao Huahong , Tan Zhican. Determination of Curcumin in Plasma by LC-MS/MS[J]. Chinese Pharmaceutical Affairs, 2012, 26(11): 1246-1248 | |
| Authors: | Luo Wenhui Sun Dongmei Gao Huahong Tan Zhican |
| Affiliation: | 1,3(1Guangdong Provincial Institute of Traditional Chinese Medicine,Guangzhou 510095;2Sanya Hospital of TCM,Hainan Province;3Guangzhou University of Chinese Medicine) |
| Abstract: | Objective To establish an LC-MS/MS method to determine the content of curcumin in plasma.Methods A high performance liquid chromatography-electrospray mass spectrometry method was utilized for rapid determination of curcumin in plasma.The analysis was carried on a Hypersil GOLD C18 column.The column temperature was 35 ℃,with linear gradient elution using water and methanol,and the flow rate was 0.2 mL·min-1.The quantitative analysis was done by selected reaction recording（SRM）mode of heater electrospray ionization（HESI）.The plasma sample was deposited by acetonitrile directly.Results A good linearity was detected in the linear range of 2.5-187.5 ng·mL-1 for curcumin;both intra-day and inter-day RSD were less than 15%（n=5）.Conclusion The method is simple,sensitive,rapid and suitable for therapeutic drug monitoring and pharmacokinetic study. |
| Keywords: | LC-MS/MS curcumin plasma concentration |
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