| HPLC法测定扁咽口服液中盐酸小檗碱的含量 |
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| 引用本文： | 李翔,刘皈阳,马建丽,王晓青,高磊. HPLC法测定扁咽口服液中盐酸小檗碱的含量[J]. 中国药事, 2012, 26(11): 1235-1237 |
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| 作者姓名： | 李翔 刘皈阳 马建丽 王晓青 高磊 |
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| 作者单位： | 解放军总医院第一附属医院,北京,100048 |
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| 摘 要： | 目的建立HPLC法测定扁咽口服液中盐酸小檗碱的含量。方法采用高效液相色谱法,色谱柱：Agilent Eclipse XDB-C18;流动相：甲醇-20mM醋酸铵溶液（40∶60）;流速：1.0mL.min-1;检测波长：345nm;柱温：30℃;进样量：10μL。结果盐酸小檗碱浓度在2.712～271.2μg.mL-1范围内与其峰面积线性关系良好（r=0.9999）,平均回收率为99.31%,RSD为1.35%（n=6）。结论本方法快速、简便、准确,可用于扁咽口服液的质量控制。
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| 关 键 词： | 扁咽口服液 盐酸小檗碱 高效液相色谱法 含量测定 |
Determination of Berberine in Bianyan Oral Solution by HPLC |
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| Li Xiang , Liu Guiyang , Ma Jianli , Wang Xiaoqing , Gao Lei. Determination of Berberine in Bianyan Oral Solution by HPLC[J]. Chinese Pharmaceutical Affairs, 2012, 26(11): 1235-1237 |
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| Authors: | Li Xiang Liu Guiyang Ma Jianli Wang Xiaoqing Gao Lei |
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| Affiliation: | (First Affiliated Hospital,General Hospital of PLA,Beijing 100048) |
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| Abstract: | Objective To establish an HPLC method for determination of berberine in Bianyan oral solution.Methods The Agilent Eclipse XDB-C18 was used with the mobile phase consisted of methanol-20 mM ammonium acetate（40∶60）;the flow rate was 1.0 mL·min-1;the detection wavelength was 345 nm;the column temperature was set at 30 ℃;the inject volume was 10 μL.Results A good linear correlation of berberine was observed within the range of 2.712-271.2 μg·mL-1（r=0.9999）.The average recovery was 99.31% with RSD of 1.35%（n=6）.Conclusion The method is rapid,simple and accurate,which can be used for the quality control of Bianyan oral solution. |
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| Keywords: | Bianyan oral solution berberine HPLC determination |
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