愈疡散对胃溃疡大鼠血一氧化氮和内皮素的影响

目的]探讨愈疡散对胃溃疡大鼠血一氧化氮(NO)和内皮素-1(ET-1)的影响.方法]采用大鼠冰醋酸胃溃疡模型,设空白对照组(A组)、假手术组(B组)、西药对照组(C组)、愈疡散大、小剂量组(D、E组)、胃溃疡模型组(F组).A、B、F组均灌服0.85%氯化钠溶液;D、E组分别灌服愈疡散5.0g/kg体重、2.5g/kg体重;C组灌服雷尼替丁0.03g/kg体重.于治疗前后分别检测血NO及ET-1.实验结束后,用苏木精-伊红染色对大鼠再生胃黏膜厚度、上皮细胞/腺腔数比值进行观察.结果]造模7 d,C、D、E、F组血清NO显著降低,血浆ET-1明显升高,与A、B组比较差异有统计学意义(P＜0.05).治疗28 d,D组血清NO显著升高,而血浆ET-1明显降低,与F、E组比较均P＜0.05.再生胃黏膜厚度:D、E、C组明显高于F组(P＜0.05),D、E组与A、B组比较均P＜0.05.上皮细胞/腺腔数比值:A、B组与C、F组,D组与C、F组之间均P＜0.05.结论]愈疡散促进溃疡愈合,增加胃黏膜的防御能力,提高再生黏膜功能成熟度,可能与其诱导、促进NO合成,反馈性地抑制ET-1释放,维持NO和ET-1的动态平衡有关.

Effect of yuyang powder on nitric oxide and endothelin in rats with gastric ulcer

bjective] To explore the effect of Yuyang powder (YP) on nitric oxide(NO) and endothelin(ET-1) in rats with gastric ulcer. Methods] Rats with gastric ulcer were induced by icy acetic acid. All the rats were randomly divided into 6 groups, namely normal group (group A) , sham -operation group ( group B) , ranitidine group ( group C) , high-dosage YP group ( group D ) , low-dosage YP group(group E) , and model group(group F). Rats in group A, B, F were fed with solution of 0. 85% sodium chloride, those in group B and E were fed with YP suspension by 5 g/kg weight and 2. 5 g/kg weight respectively, those in group C with ranitidine by 0. 03 g/kg/weight. Contents of NO and ET-1 in the blood were determined before and after the treatment. After the experiment the thickness of regenerated gastric mucosa of rats with gastric ulcer, the ratio of epithelial cells and the number of gland lumen were measured and compared by haematoxylin eosin ( HE) staining. Results] Seven days after the model inducement, in group C, D, E, F the serum content of NO significantly declined and the plasma content of ET-1 significantly increased, there was significant difference compared with those in group A and B ( P <0. 05). Twenty - eight days after the treatment, the serum content of NO in group D increased significantly, but the plasma content of ET-1 declined significantly, there was significantly difference compared with those in group E and F (P <0. 05). The thickness of regenerated gastric mucosa in group D, E, C was significantly higher than that of group F ( P < 0. 05 ) . There was significant difference between group D, E and group A, B ( P <0.05). As for the ratio of epithelial cells and the number of gland lumen, there was significant difference between group A, B and group C, F ( P <0.05). There was significant difference between group D and group C, F ( P <0. 05). Conclusion] YP could accelerate the healing of gastric ulcer, promote defensive function of gastric mucosa, and highten the degree of functional maturation of regenerated mucosa, which may relate to inducing and promoting the synthesis of NO, inhibiting the release of ET-1 with feedback and maintaining the dynamic balance of No and ET-1.