血浆制备时间和速冻方法对冷沉淀凝血因子质量的影响

目的探讨新鲜血浆的制备时间和速冻方法对冷沉淀凝血因子质量的影响,选择合适的制备时间和速冻方法。方法随机抽取制备时间分别为2 h、4 h、6 h、8 h新鲜血浆各16人(份),采用无菌接驳机平均分为A、B两实验组,分别用平板速冻机和传统低温冰箱速冻制备新鲜冰冻血浆(FFP);在相同条件下分别对两组FFP制备冷沉淀;采用凝固法检测两组冷沉淀的凝血因子Ⅷ(FⅧ)和纤维蛋白原(Fg)。结果血浆制备时间为2 h、4 h、6 h、8 h的冷沉淀FⅧ活性(IU)A组分别为78.40±22.87、74.06±23.72、71.25±19.93、70.53±18.84,B组分别为66.60±17.12、58.08±18.19、52.57±12.26、51.19±12.51。A、B组冷沉淀FⅧ随着制备时间的延长,均呈下降趋势,相同制备时相A与B组比较均有统计学差异(P<0.05);A组FⅧ活性4个制备时相间比较差异无统计学意义(P>0.05),B组2 h与6 h、8 h比较差异均有统计学差异(P<0.05)。血浆制备时间为2 h、4 h、6 h、8 h的冷沉淀中Fg含量(mg)A组分别为114.53±24.76、117.62±27.61、114.44±22.84、120.23±26.48,B组分别为113.36±23.53、116.43±25.38、115.28±23.66、117.92±25.58,Fg含量在不同制备时间和速冻方法条件均无统计学差异。结论 8 h内制备血浆2种速冻方法均能满足冷沉淀质量要求,平板式速冻机制备血浆的冷沉淀FⅧ活性显著性高于传统低温冰箱。

Influence of time and temperature on coagulation factor in cryoprecipitate.

Objective To investigate the effects of time and temperature on coagulation factor in cryoprecipitate. Appropriate preparation time and quick freezing method were chosen. Methods Sixty - four plasma units preparated period of 2 h, 4 h, 6 h or 8 h before plasma freezing were divided into two equal units. One unit was frozen in flat frozen machine（ A group）, and the other unit was frozen in cryogenic refrigerator （ B group） . The cryoprecipitates prepared from fresh frozen plasma by siphonage method thawing at 4℃ water bath. FⅧ, fibrinogen （Fg） in cryoprecipitates were measured by coagulometer （ STA - Compact ）. Results The activity of FⅧ in A group at 2 h, 4 h, 6 h or 8 h were 78.40 ± 22.87, 74.06 ± 23.72, 71.25 ± 19.93, 70.53 ± 18.84. The activity of FⅧ in B group were 66.60 ± 17.12, 58.08 ± 18.19, 52.57 ± 12.26, 51.19 ± 12.51, respectively. The activity of FⅧ in A group and B group was obviously decreasing trend following the preparation with extension of time. The FV$ activity in four preparation period were compared. The activity of group A was not statistically significant （ P 〉 0.05 ）. In B group, it was significantly different between 2 h and 6 h , 8 h （ P 〈 0. 05 ）. The concentration of Fg in A group at 2, 4, 6 or8 hours were 114.53 ± 24.76, 117.62 ± 27.61, 114.44 ± 22.84, 120.23 ± 26.48, and B group were 113.36 ± 23.53, 116.43 ± 25.38, 115.28 ± 23.66, 117.92 ± 25.58. The concentration of Fg was not significantly different between preparation time and quick freezing method. Conclusion Two frozen methods prepared plasma in 8 hours. These methods could meet quality requirements of cryoprecipitate. The plasma frozened in flat frozen machine will be more preferable than in cryogenic refrigerator