重型丙型肝炎肝外组织丙型肝炎病毒感染状态免疫组化研究

目的　探讨丙型肝炎患者肝外组织丙型肝炎病毒（ＨＣＶ） 感染状态、致病性及其意义。方法　采用免疫组化法检测９ 例重型丙型肝炎患者肾、心、胰腺和肠等肝外组织内ＨＣＶ 多种抗原表达，并观察各组织病理改变。结果　９ 例中６ 例（６６ ．７ ％ ） 在肝外组织检出ＨＣＶ 抗原。抗原定位于细胞浆内，呈均质型、包涵体型和膜下型。组织内抗原表达阳性细胞数量较少，不同组织间阳性细胞种类、数量和分布略有差异。病理学观察未发现细胞内ＨＣＶ 抗原阳性表达与组织病变有明确相关性。结论　ＨＣＶ 可以感染肝外组织，但未显示显著致病性。作为致病因子的ＨＣＶ 持续存在肝外多种脏器细胞内，在丙型肝炎慢性化和干扰素治疗后病情复发中可能起重要作用

Immunohistochemical study on the state of extrahepatic hepatitis C virus infection in severe hepatitis C

Objective To explore the state of extrahepatic hepatitis C virus (HCV) infection, viral pathogenicity and its significance in hepatitis C patients.Methods Immunohistochemical assay was used to examine the expression of various HCV antigens in the kidney, heart, pancreas and intestine from 9 severe hepatitis C patients. Relationship between the expression of HCV antigens and tissue lesions was evaluated.Results HCV antigens in extrahepatic tissues were detected in 6 of 9 (66.7%) hepatitis C patients and the expression of HCV antigens was observed only within cytoplasm in the style of homogenous, inclusion and submembrane distribution. The amount of positive staining cells was less in the tissue and there were a few differences of kind, amount and distribution of HCV antigens positive staining cells among different extrahepatic tissues. There was no relationship between the expression of HCV antigens and tissue lesion.Conclusions These results show that HCV exists in different extrahepatic tissues, but do not bring out obvious cell injury. As a etiologic agent, the persistence of HCV in extrahepatic tissues may take an important role in the relapse as well as the chronicity of hepatitis C after discontinuation of interferon therapy.