河南省人免疫缺陷病毒流行株的基因亚型分析

目的　研究流行于河南省献血员中的人免疫缺陷病毒（ＨＩＶ） 外膜蛋白（ｅｎｖ） 基因亚型。方法　提取ＨＩＶ 感染者外周血单个核白细胞（ＰＢＭＣ） 的ＤＮＡ，经套式聚合酶链反应（ＮｅｓｔｅｄＰＣＲ） 扩增ｅｎｖ 基因片断，对Ｃ２Ｖ３ 区３５０ ～４５０ 核苷酸顺序进行测定和分析。结果　２０ 份样品的ｅｎｖ 基因序列与Ｂ亚型最为接近，基因离散率为８ ．３４ ％ ，与其它国际亚型的基因离散率在２０ ％ 以上；与流行于云南的Ｂ 亚型毒株基因离散率为４ ．１８ ％ ；样品间的基因离散率为３ ．４ ％ 。结论　流行于河南省献血员中的ＨＩＶ 属Ｂ亚型，并与云南的流行株密切相关；其在河南的流行时间在３ ～６ 年间。

Genomic subtypes of HIV-1 in Henan province

Objective Toidentify HIV 1 envelope sequence subtypesin infected blood donorsfrom Henan province. Methods DNAextracted from PBMCwere amplifiedin the presence ofenvfragment by nested PCR.The C2 V3 region was sequenced and a phylogenetic tree was constructed . Results The overallenv gene divergenceis8 .34 % between samples with HIV 1 Bsubtype,and 4 .18 % with reference strains of Yunnan origin .Thesample gene divergence with othersubtypes A,C,D,Eis over 23 % .The innergroup gene divergenceofsamplesis3 .4 % .The globally predominant GPGQsequenceatthetip of V3 motifisfoundin 11 strains (55 % ) ,typical European GPGRsequence were found in 7 strains (35 % ) . Twostrains contain a GPGKsequence. Conclusion These findings suggest the epidemic ofsubtype Bof HIV 1 in Henan province anditisclosely related tothoseidentifiedin Yunnan province.