原发性肝癌和癌旁组织中NK细胞受体表达及意义

目的:通过研究原发性肝癌和癌旁组织中NK细胞表面受体活化性及抑制性受体的表达,分析探讨这两种受体含量变化在原发性肝癌发生发展中的关系及其临床价值。方法:通过流式细胞术及免疫组织化学方法检测52例原发性肝癌组织及其癌旁组织中NK细胞数及其活化性、抑制性受体的表达,并结合临床相关因素进行统计学分析。结果:原发性肝癌的NK细胞数量明显低于癌旁对照组(P<0.01),原发性肝癌组织活化性受体NKG2D、NKP44明显低于癌旁组织(P<0.05),而抑制性受体CD158b、CD159a明显高于癌旁组织(P<0.05)。NKG2D、NKP30、NKP44的表达与肝癌临床分期负相关,即在早中期的患者含量较高,越晚期患者肝癌组织中NKG2D、NKP30、NKP44含量越低(P<0.05),NK细胞中抑制性受体CD158b、CD159a的含量在越晚期患者肝癌组织中含量越高(P<0.05)且与AFP高低有关及HBsAg的感染有联系。而NK受体的表达在是否有远处转移、肿瘤分化程度之间以及不同的病灶大小之间差异无统计学意义(P>0.05)。结论:原发性肝癌发病可能与NK细胞减低及NK细胞活化性受体表达降低、抑制性受体表达升高有关。

Expression and significance of the NK cell receptors in primary hepatocellular carcinoma and paracancerous tissues

AIM: To investigate the expression of the activating and inhibitory receptors on the surface of NK cells of primary hepatocellular carcinoma and its adjacent tissues,and the relationship between these two receptors and occurrence and development of primary liver cancer was analyzed.METHODS: The number and activity of the NK cells,the expression of the activating and the inhibitory receptors on the surface of those cells were detected flow cytometry and immunohistochemistry,which were obtained from 52 cases of primary hepatocellular carcinoma and its adjacent tissues.The relative analysis was done between those results and clinical relative factors.RESULTS: In the tissues of primary hepacellular carcinoma,the number of NK cells is lower than that in the adjacent tissues obviously(P<0.01);the expression of activating receptos,NKG2D and NKP44,is also lower than that in the adjacent tissues obviously(P<0.05);the expression of inhibitory receptors,CD158b and CD159a,is significantly higher than that in the adjacent tissues(P<0.05).A negative correlation was found between the expression of NKG2D,NKP30 and NKP44 and the clinical stage of the liver cancer.The expression of NKG2D,NKP30 and NKP44 was higher in patients with early and middle stages(P<0.05).The content of the inhibitory receptors of NK cells,CD158b and CD159a,is higher in tissues from partients with advanced cancer stage(P<0.05).That’ s also correlated with the level of AFP and the HBsAg.There is no significant statistical difference between the expression of NK receptors and the distant metastasis,tumor differentiation as well as the tumor size(P>0.05).CONCLUSION: The decrease of NK cell numbers and the activating NK cell receptors and the increase of the inhibitory receptors would be relevant to the incidence of primary hepacellular carcinoma.