| 人胎肾上腺髓质细胞的原代培养 |
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| 引用本文： | 何旦莎,邓维意,李元浩,曾庆荣,叶劲. 人胎肾上腺髓质细胞的原代培养[J]. 解剖学报, 1992, 23(4): 380-383 |
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| 作者姓名： | 何旦莎 邓维意 李元浩 曾庆荣 叶劲 |
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| 作者单位： | 广州医学院神经科学研究所，广州医学院神经科学研究所，广州医学院神经科学研究所，广州医学院神经科学研究所，广州医学院神经科学研究所 广州 510182，广州 510182，广州 510182，广州 510182，广州 510182 |
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| 摘 要： | ![]()
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| 关 键 词： | 肾上腺 随质细胞 细胞培养 人胎 |
PRIMARY CULTURE OF HUMAN FETAL ADRENAL MEDULLARY CELLS |
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| He Dansha,Deng Weiyi,Li Yuanhao,Zeng Qingrong,Yie Jing. PRIMARY CULTURE OF HUMAN FETAL ADRENAL MEDULLARY CELLS[J]. Acta Anatomica Sinica, 1992, 23(4): 380-383 |
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| Authors: | He Dansha Deng Weiyi Li Yuanhao Zeng Qingrong Yie Jing |
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| Abstract: | ![]()
A simple method for culture of human fetal adrenal medullary cells is described. Enzymatically dissociated human fetal adrenal cells were isolaled by centrifugation on Percoll in the buffer. Adrenal medullary cells with 75%-85% purity were obtained by this procedure. The medullary cells were then plated on plastic culture dishes coated with collagen. After 2-7 days medullary Cells began to flatten and extended processes. A further identification of the cells as medullary in origin was confirmed, by specific catecholamine fluorescence technique. Catecholamine content in the medium was also determined by HPLC. |
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| Keywords: | Adrenal medullary cells Cell culture Human fetus |
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