丁基苯酞对缺血脑组织中及低糖低氧刺激后培养的神经细胞中胆碱乙酰化酶活性的影响

 目的:观察丁基苯酞(dl-NBP),d-NBP和l-NBP对脑缺血后皮层和纹状体中以及低糖低氧和NMDA刺激后培养的胎鼠皮层神经元中胆碱乙酰化酶活性变化的影响?方法:大脑中动脉堵塞起始处造成大鼠局灶性脑缺血;胎鼠皮层神经元以低糖低氧培养基培养造成低糖低氧模型;胆碱乙酰化酶活性以分光度法进行测定?结果:脑缺血后缺血区皮层和纹状体中胆碱乙酰化酶活性分别下降了61.3%和58.4%?dl-NBP,d-NBP和l-NBP(10和20mg·kg^-1,ip)于脑缺血后5和60min给药2次皆可显著提高脑组织中胆碱乙酰化酶活性?dl-NBP,d-NBP和l-NBP(0.1～10μmol·L^-1)对低糖低氧及NMDA刺激后神经细胞中胆碱乙酰化酶活性也有明显的提高作用?结论:dl-NMP改善局灶性脑缺血后动物的学习记忆功能可能与其对胆碱能神经功能的改善有关?

Effect of dl-3-n-butylphthalide on the activity of the choline acetyltransferase in ischemic brain and cultured neurons subjected to hypoglycemia/hypoxia

OBJECTIVE: To investigate the effects of dl-3-n-butylphthalide (dl-NBP),d-NBP and l-NBP on the activity of choline acetyltransferase (ChAT) in ischemic brain after focal cerebral ischemia and in neurons subjected to hypoglgcemia/hypoxia. METHODS: Focal cerebral ischemia was induced by blockade of the origin of middle cerebral artery (MCAO) and perinatal cortical rat neurons was cultured with hypohlycemia/hypoxic medium to induce hypoglycemia/hypoxia injury.The activity of ChAT was determined spectrophotometrically. RESULTS: The results indicated that the activity of ChAT was decreased by 61.3% and 58.4% respectively in cerebral cortex and striatum after six hours of MCAO.dl-NBP,d-NBP and l-NBP (10 and 20mg·kg^-1,ip) were shown to increase ChAT activity in ischemic brain significantly.The decreased activity of ChAT in cultured perinatal rat cortical neurons induced by hypoglycemia/hypoxia or by NMDA was also improved by dlNBP,dNBP and lNBP (0.1～10mol·L^-1). CONCLUSION: The effect of dl-NBP on learning and memory function impaired by focal cerebral ischemia may be related to its protective effect on the activity of choline acetyltransferase.