双色荧光原位杂交技术检测浆细胞白血病13号染色体缺失

为了解浆细胞白血病（PCL）中13号染色体缺失情况及其与临床特征的相关性，运用双色荧光原位杂交（FISH）技术及位于13q14和13q31的序列特异性DNA探针，对21例初发PCL患者的间期细胞进行13号染色体缺失的检测。结果显示，21例PCL中13例（61．9％）具有del（13q14）异常，其阳性细胞率在52％-98％之间；12例（57．4％）有del（13q31）异常，其阳性细胞率在50％-98％之间。13例del（13q14）异常患者中，12例伴有del（13q31），仅1例阴性。结论：PCL中13号染色体缺失发生率较高，大部分PCL患者13号染色体缺失片段可能涉及由13q14到13q31的整个区间。FISH技术是一种在分析PCL13号染色体缺失异常方面较为快速、准确和敏感的方法。

Detection of Chromosome 13 Deletion in Plasma Cell Leukemia by Dual-color Fluorescence in Situ Hybridization

This study was aimed to investigate the deletion features of chromosome 13 in plasma cell leukemia(PCL) and its relationship with clinical features.The dual-color fluorescence in situ hybridization(FISH) with two different specific probes for the regions containing 13q14 and 13q31 was used to delect the chromosome in 21 patients with PCL.The results showed that 13(61.9%) out of 21 PCL had 13q14 deletion.The number of 13q14 deletion cells ranged from 52% to 98%.12 patients(57.4%) had 13q31 deletion.The number of cells with 13q31 deletion ranged from 50% to 98%.Out of 13 cases with(13q14) delection,12 cases had(13q31) delection,but only 1 out of 12 cases was negative.It is concluded that chromosome 13 deletion may be involved in initiation of a subset of PCL,and the region of loss at chromosome 13 locates between 13q14 and 13q31 in PCL.FISH is a more rapid,exact and sensitive technique in analysis of chromosome 13 deletion in PCL.