真核细胞翻译起始因子4E蛋白在子宫内膜癌中的表达及意义

目的半定量分析子宫内膜癌及正常子宫内膜组织中真核细胞翻译起始因子4E(eIF4E)蛋白的表达特点及其临床意义。方法采用SP免疫组化法检测eIF4E蛋白在40例子宫内膜癌组织和22例正常子宫内膜组织中的表达,使用Image pro-plus 6.0专业图像分析软件半定量分析eIF4E蛋白表达的阳性强度。结果子宫内膜癌组织及正常子宫内膜组织中eIF4E蛋白表达平均光密度值(MOD值)分别为0.189±0.059和0.108±0.021,两者MOD值差异有统计学意义(P<0.01)。eIF4E表达与组织学分级、FGIO分期及淋巴转移显著相关(P<0.05),而与年龄、病理类型及浸润深度无关(P>0.05)。结论 eIF4E蛋白与子宫内膜癌的发生发展及生物学行为关系密切,可作为子宫内膜癌恶性改变的分子标志。

Semi-quantitative analysis and clinical significance of eIF4E protein in endometrial carcinoma

Objective To investigate the expression and clinical signification of eIF4E protein in endometrial carcinoma.Methods In 40 cases of endometrial carcinoma and 22 cases of normal endomedium,the expression of eIF4E protein detected by immunohistochemistry of SP were analyzed semi-quantitatively by Image pro-plus 6.0 image analysis system.Results The mean optical densities(MODs) of eIF4E protein in endometrial carcinoma and normal endomedium were 0.189±0.059 and 0.108±0.021,respectively,both normally distributed.The expression of eIF4E protein in endometrial carcinoma was significantly higher than that in normal endomedium(P<0.01),and positively correlated with differentiation degree,FIGO stage and differentiation degree(P<0.05).Conclusion The expression of eIF4E is positively associated with development,progression and clinicopathologic features of endometrial carcinoma;suggesting a novel prognostic factors for endometrial carcinoma.