小型猪一侧失牙及义齿修复后TMJ关节液中IL-1β、TNF-α、TGF—β1含量的变化

目的：检测小型猪一侧失牙及义齿修复后颞下颌关节（temporomandibularjoint，TMJ）关节液中IL-1β、TNF-α、TGF-β1含量，探讨其在颞下颌关节紊乱病中的作用及相互关系。方法：11头小型猪随机分为空白组2头、拔牙组4头和修复组5头，拔牙组和修复组左侧后牙全部拔除，修复组于拔牙后3个月义齿修复；每月采集双侧TMJ关节液，ELISA法测定IL-1β、TNF-α、TGF-β1含量（共6个月）。结果：拔牙组和修复组关节液中IL-1p浓度于第1个月达到最大值，拔牙组于第3个月、修复组于第5个月IL-1β浓度达到最小值；拔牙组于第4个月、修复组于第3个月TNF-α达到最大值；拔牙组和修复组于第3个月TGF-β1浓度达到最大值。结论：IL-1β启动并与TNF—α协同破坏TMJ；TGF-β1在TMJ损伤的修复中发挥重要作用。

Fluctuation of IL-1β、TNF-α、TGF-β1 in synovial fluid from TMJs in miniature pigs following one side posterior teeth missing and denturte restoration

Objective： To simultaneously detect the levels of IL-1β,TNF-α,TGF-β1 in synovial fluid from temporomandibular joints （TMJs） in miniature pigs follwing one side posterior teeth missing and denture restoration, investigate their roles and interrelationships in temporomandibular disorders （TMD）. Methods： 11 miniature pigs were randomly divided into 3 groups： group Ⅰ- control group （2 pigs）, group Ⅱ （4 pigs）, group Ⅲ （5 pigs）. In group Ⅱ and Ⅲ, pigs＇ left side posterior teeth were pulled out. Dentures were fabricated for group Ⅲ3 months after teeth missing. Synovial fluid samples were harvested monthly for 6 months, Sandwich-ELISA was used to examine the concentration of cytokines. Results： The level of IL-1βin group Ⅱ and Ⅲ increased to the peak at the 1St month, and decreased to the minimum （group Ⅱ -at the 3rd month, group Ⅲ -at the 5rd month）; TNF-α couldn＇t be detected until the 3rd month and reached its crest （group Ⅱ -at the 4rd month, group Ⅲ-at the 3rd month）; The concentration of TGF-β1 in group Ⅱ and Ⅲ rised to the maximum at the 3rd month. Conclusion： IL- 1β initiates the pathological process of TMD and cooperates with TNF-αto injure TMJ; TGF-β1 may play an important role in repairing the damaged TMJ.