| 双波长HPLC同时测定黄连及金芪降糖片中6种生物碱的含量 |
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| 引用本文： | 陈芳,邓雁如,许妍妍,夏娜,高璎.双波长HPLC同时测定黄连及金芪降糖片中6种生物碱的含量[J].中国实验方剂学杂志,2013,19(17):60-65. |
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| 作者姓名： | 陈芳 邓雁如 许妍妍 夏娜 高璎 |
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| 作者单位： | 天津中医药大学,天津市中药化学与分析重点实验室,天津300193 |
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| 基金项目： | 国家自然科学基金项目(81073033);国家重点基础研究发展计划项目(2009CB523003) |
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| 摘 要： | 目的:建立HPLC同时测定黄连及金芪降糖片中木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱6种生物碱含量的方法.方法:Waters Symmetry ShieldTM RP18色谱柱(4.6mm×250mm,5μm),流动相乙腈(0.3％甲酸和0.3％三乙胺)(A)-水(0.3％甲酸和0.3％三乙胺)(B)梯度洗脱,体积流量1.0 mL· min-1,检测波长345,270 nm,柱温25℃.结果:木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀及盐酸小檗碱线性范围分别为0.012 06 ～0.241 2,0.038 24 ～0.7648,0.088 02 ～1.760 4,0.016 55～0.331 0,0.036 08～0.721 6,0.189 41 ～3.788 2μg(r≥0.999 8),平均加样回收率95％～105％.结论:方法专属性强、灵敏度高、重复性好、简便易行,可用于同时测定黄连及金芪降糖片中木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量,可为金芪降糖片的现代制剂研究和质量控制提供依据.
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| 关 键 词： | 金芪降糖片 黄连 木兰花碱 表小檗碱 黄连碱 盐酸药根碱 盐酸巴马汀 盐酸小檗碱 |
| 收稿时间： | 2012/10/19 0:00:00 |
Simultaneous Determination of Six Alkaloids in Coptidis Rhizoma and Jinqi Jiangtang Tablets by Double Wavelengths and HPLC |
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| CHEN Fang,DENG Yan-ru,XU Yan-yan,XIA Na and GAO Ying.Simultaneous Determination of Six Alkaloids in Coptidis Rhizoma and Jinqi Jiangtang Tablets by Double Wavelengths and HPLC[J].China Journal of Experimental Traditional Medical Formulae,2013,19(17):60-65. |
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| Authors: | CHEN Fang DENG Yan-ru XU Yan-yan XIA Na and GAO Ying |
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| Institution: | Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | Jinqi Jiangtang Tablets Coptidis Rhizoma magnoflorine epiberberine coptisine jatronhizine palmatine berberine |
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