| ￣3H─TdR法检测LAK细胞活性的建立及人肺腺癌细胞株（GLC─82）对LAK细胞的敏感性 |
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| 引用本文： | 康世均,李金瀚,王小宁.￣3H─TdR法检测LAK细胞活性的建立及人肺腺癌细胞株（GLC─82）对LAK细胞的敏感性[J].癌症,1995(2). |
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| 作者姓名： | 康世均 李金瀚 王小宁 |
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| 作者单位： | 第一军医大学南方医院肿瘤科，第一军医大学免疫学教研室 |
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| 摘 要： | 用３Ｈ－ＴｄＲ释放法检测了个旧人肺腺癌细胞株（ＧＬＣ─８２），小鼠肥大细胞瘤（Ｐ８１５）及人红白血病细胞株（Ｋ５６２）对自然杀伤细胞（ＮＫ细胞），淋巴因子激活的杀伤细胞（ＬＡＫ细胞）的敏感性。结果表明：用３Ｈ─ＴｄＲ释放法检测ＮＫ、ＬＡＫ细胞活性具有灵敏、准确、重复性好的优点，适用于国内一般实验室开展；ＧＬＣ─８２细胞对ＮＫ细胞不敏感，而对ＬＡＫ细胞敏感，提示人肺腺癌用ＬＡＫ细胞治疗可能有效，ＧＬＣ─８２细胞可作为检测ＬＡＫ细胞活性的靶细胞。
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| 关 键 词： | 肺癌，3H─TdR，LAK细胞，NK细胞 |
THE DETECT1ON OF LAK CELL ACTIVITY WITH 3H-TDR RELEASE ASSAY AND
SENSITIVITY OF GLC-82 CELL(HUMAN LUNG ADENOCARCINOMA)TO LAK CELL |
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| KANG Shi-junLI Jin-han WANG Xiao-ning.THE DETECT1ON OF LAK CELL ACTIVITY WITH 3H-TDR RELEASE ASSAY AND
SENSITIVITY OF GLC-82 CELL(HUMAN LUNG ADENOCARCINOMA)TO LAK CELL[J].Chinese Journal of Cancer,1995(2). |
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| Authors: | KANG Shi-junLI Jin-han WANG Xiao-ning |
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| Abstract: | The sensitivity of GLC-82,P815 and K562 cell line to
lymphokineactivated killer cells(LAK cells)and natural killer
cells(NK cells)was detected with 3H-TdR release assay.The results
show that 3H-TdR release assay to detect NK and LAKcells activity is
suceptible,accurate, repeated and adopted in common
laboratory.GLC-82cell line is suceptible to LAK cells but not to NK
cells,which suggest that it may beeffect that human lung
adenocarcinoma treated with LAK cells. |
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| Keywords: | H-TdR LAK cellsNK cells Lung adenocarcinoma |
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