大黄素对肺成纤维细胞增殖及细胞周期的影响

目的　探讨大黄素对体外培养肺成纤维细胞 (L F)增殖的作用。方法　采用体外细胞培养、透射电镜、流式细胞仪和同位素检测等技术 ,比较了在大黄素作用下 L F的增殖 ,并检测大黄素作用下 L F的细胞增殖周期和电镜下的形态学特征。结果　大黄素能够抑制体外培养 L F的增殖 (P<0 .0 1) ,并随着剂量增加抑制效应增强 ,大黄素可以提高 L F细胞周期中 G1 期细胞的比例 (P<0 .0 1) ,减少 S期和 G2 期细胞的比例 (P<0 .0 1)。同时 ,发现大黄素作用组的 L F形态上与对照组无明显差异。结论　大黄素具有抑制 L F增殖和阻止 L F由 G1 期进入 S期和 G2期的作用 ,同时 ,本研究的最大剂量大黄素 (10 0 μg/ ml)对 L F细胞形态无明显损伤。

Effects of Emodin on Lung Fibroblast Proliferation and Cell Cycle in vitro

OBJECTIVE: To gain a better understanding of the effect of emodin on lung fibroblast (LF) proliferation in vitro. METHODS: The methods of cell cultured in vitro, 3H-TdR incorporation, flow cytometry and electron microscopy were used in research to compare LF proliferation, cell cycle and morphologic change of LF in vitro. RESULTS: It was found that emodin reduced the proliferation of LF in vitro (P < 0.01), and such inhibitory effect was dose-dependent. Emodin delayed the progression of LF from G1 to S and G2 phase (P < 0.01). Also, electron microscopic examination demonstrated that the ultrastructure of LF in emodin group was not markedly worse as compared with that in the control group. CONCLUSION: Emodin inhibits the proliferation of LF and delays the progression of LF cell cycle. Emodin at the dose of 100 micrograms/ml does not destroy the ultrastructure of LF cells. These findings may provide a theoretical basis for the endeavors to use emodin for preventing lung fibrosis diseases.