防紫外线损伤的植物提取液紫外可见光谱研究

目的开发源自植物的天然紫外线吸收剂,用于紫外线损伤的防护。方法对玉竹、洋葱的水提液、醇提液分别进行紫外可见光谱研究,并将黑龙江玉竹和湖南玉竹的提取液进行光谱比较,将1g黑龙江玉竹、湖南玉竹分别进行水提取和醇提取,将稀释5倍的水提液和醇提液原液,进行紫外可见光谱扫描。将20g鲜洋葱的水提液和醇提液稀释50倍,进行紫外可见光谱扫描。结果黑龙江和湖南玉竹水提液在290～400nm均有强吸收,在中波紫外线区(290～320nm),黑龙江玉竹的吸收值略低于湖南玉竹,在长波紫外线区(320～400nm),黑龙江玉竹水提液的吸收略优于湖南玉竹。黑龙江和湖南玉竹醇提液在290～400nm的吸收强度差异明显,在中波紫外线区(290～320nm),湖南玉竹醇提液有强吸收,黑龙江玉竹醇提液吸收明显显弱。洋葱的水提液仅在358nm处有强吸收,洋葱醇提液在290～400nm均有强吸收。结论玉竹水提液对中长波紫外线吸收能力明显强于醇提液。洋葱醇提液在中长波紫外线区有广谱强吸收。

UV-visible spectrum study on anti-UV damaging plants extraction

Objective To develop nature agent absorbing ultraviolet from plants for preventing UV damage.Methods Conduct ultraviolet visible spectrum scanning to aqueous extraction and alcohol extraction from polygonatum odoratum and onion.And compare the spectrum of Heilongjiang and Hunan polygonatum odoratum extraction.Dilute 5 times of the aqueous and alcohol extraction from 1g Heilongjiang,Hunan polygonatum odoratum to conduct UV visible spectrum scanning.Dilute 50 times of the aqueous and alcohol extraction of 20 g fresh onion and conduct ultraviolet visible spectra scanning.Results For the aqueous extraction of Heilongjiang and Hunan polygonatum odoratum by ultraviolet visible spectrum scan,both of them had strong absorption in the 290nm to 400nm while the absorption value of Heilongjiang's is slightly lower than the Hunan's in the ultraviolet region(290nm to 320nm) and the absorption value of Heilongjiang's was significantly better than Hunan's in the long-wavelength ultraviolet region(320nm to 400nm).For alcohol extraction,the absorption values were significantly different from 290nm to 400nm.Hunan's was stronger while Heilongjiang's was weaker in 290-320nm.Onion aqueous extraction had strong absorption only at 358nm while alcohol extraction has strong absorption from 290nm to 400nm.Conclusion absorption value of polygonatum odoratum aqueous extraction was stronger than alcohol extraction.Onion alcohol extraction in medium and long wave ultraviolet region had strong absorption spectrum.