| 一种适合膜片钳记录的成年大鼠背根神经节细胞培养方法 |
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| 引用本文： | 刘晓红,曾俊伟,赵延东,阮怀珍.一种适合膜片钳记录的成年大鼠背根神经节细胞培养方法[J].遵义医学院学报,2009,32(2):115-117. |
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| 作者姓名： | 刘晓红 曾俊伟 赵延东 阮怀珍 |
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| 作者单位： | 1. 遵义医学院,生理学教研室,贵州,遵义,563003
2. 第三军医大学,基础医学部神经生物学教研室,重庆,400038 |
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| 摘 要： | 目的建立一种适合膜片钳研究的成年大鼠背根神经节（D0rsal root ganglion，DRG）细胞培养方法。方法分离并培养成年大鼠DRG神经元，显微镜下观察培养神经元的形态，膜片钳技术记录培养神经元的基本膜电生理特性及ATP激活电流。结果培养的DRG神经元形态结构完整；其静息膜电位在正常范围内，可记录到诱发动作电位及钠电流；在膜片钳全细胞记录模式下，能稳定地记录到ATP诱发电流。结论培养的成年大鼠DRG神经元适合膜片钳试验。
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| 关 键 词： | 背根神经节 细胞培养 膜片钳 |
Cultural method of dorsal root ganglion neurons of adult rat for patch clamp study |
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| LIU Xiao-hong,ZENG Jun-wei,ZHAO Yan-dong,RUAN Huai-zhen.Cultural method of dorsal root ganglion neurons of adult rat for patch clamp study[J].Acta Academiae Medicine Zunyi,2009,32(2):115-117. |
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| Authors: | LIU Xiao-hong ZENG Jun-wei ZHAO Yan-dong RUAN Huai-zhen |
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| Institution: | LIU Xiao-hong, ZENG Jun-wei, ZHAO Yan-dong, RUAN Huai-zhen (1. Department of physiology, Zunyi Medical College, Guizhou Zunyi 563003, China; 2. Department of Neurobiology, Third Military Medical University, Chongqing 400038, China) |
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| Abstract: | Objective To establish a kind of cultural method of dorsal root ganglion （DRG） neurons for patch clamp study. Methods DRG neurons of adult rat were isolated and cultured. The electrophysiological properties and ATP-induced currents of cultured DRG neurons were investigated by using patch-clamp techniques. Results The cultured cells were morphologically intact, from which evoked action potential and Na^＋ channel currents, and ATP-induced currents could be detected. Conclusions Cultured DRG neurons of adult rat are ideal for patch-clamp studies. |
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| Keywords: | dorsal mot ganglion cell culture patch-clamp |
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