| 响应曲面法优化喜树碱的超声提取工艺 |
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| 引用本文： | 康大力,;沈小钟,;张洪利. 响应曲面法优化喜树碱的超声提取工艺[J]. 西北药学杂志, 2014, 0(4): 350-353 |
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| 作者姓名： | 康大力, 沈小钟, 张洪利 |
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| 作者单位： | [1]广东食品药品职业学院,广州510520; [2]广东省中药研究所,广州510520 |
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| 基金项目： | 广东省医学基金项目（编号：B2012070） |
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| 摘 要： | 目的用响应曲面法优选喜树碱的超声提取工艺。方法以提取时间、提取温度和料液比为考察因素,用HPLC-DAD法测定喜树碱的含量,通过响应曲面法优化喜树碱超声提取工艺。结果最佳提取工艺为提取温度49℃,提取时间2.5h,料液比1∶17。结论响应曲面法筛选的喜树碱提取工艺稳定、可行。
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| 关 键 词： | 喜树碱 响应曲面法 提取工艺 |
Optimization of extraction technology for camptothecin by response surface methodology |
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| Affiliation: | KANG Dali , SHEN Xiaozhong , ZHANG Hongli (1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China; 2. Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520,China) |
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| Abstract: | Objective To optimize the extraction technology of camptothecin（CPT）by response surface methodology（RSM）.Method RSM was used to optimize the extraction process of CPT by optimizing the extraction temperature,extraction time and ratio of material to liquid.The content of CPT was determined by HPLC-DAD.Results The optimal extraction parameters were as follows：extraction temperature 49℃,extraction time 2.5hand the ratio of material to liquid 1∶17.Conclusion The established extraction technology is stable and feasible. |
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| Keywords: | camptothecin response surface methodology extraction technique |
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