氟伐他汀和辛伐他汀对人脐静脉内皮细胞黏附分子-1表达的影响

目的观察氟伐他汀和辛伐他汀对肿瘤坏死因子(TNFα)诱导的人脐静脉内皮细胞(HUVEC)血管细胞黏附分子-1(VCAM-1)表达的影响，以期探讨3-羟-3-甲基戊二酰辅酶A(HMG-CoA)还原酶抑制剂可能的非调脂抗动脉粥样硬化作用。方法体外培养HUVEC，加TNFα100U及1×10

Effect of fluvastatin and simvastatin on expression of VCAM-1 in human umbilical vein endothelial cells

Objective　To observe the influence of fluvastatin and simvastatin on TNFα-induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVEC) to investigate the non-lipid mechanisms of 3-hydroxy-3-methyl-glutaryl coenzyme A(HMG-CoA) reductase inhibitors on anti-atherosclerosis. Methods　The expression of VCAM-1 in protein level and mRNA level was detected by endothelial cell enzyme linked immunosorbent assay(ELISA), flow cytometric technique, and RT-PCR. Results　Fluvastatin and simvastatin of 1×10-8－1×10-5 mol/L at 24-hour coincubation could inhibit TNFα-induced expression of VCAM-1 in HUVEC. The inhibition of VCAM-1 expression at 12-hour and 6-hour coincubation was only found in 1×10-7－1×10-5 mol/L fluvastatin and simvastatin but not in 10-8 mol/L. The inhibitory effect was concentration-dependent and time-dependent. Conclusion　The inhibition of VCAM-1 expression in HUVEC and reduction of VCAM-1 dependent monocytes adhesion to endothelium by fluvastatin and simvastatin may crucially contribute to the clinical benefits of HMG-CoA reductase inhibitors on coronary artery disease(CAD), independent of cholesterol-lowering effect.