穴贴定喘膏体外透皮试验用皮肤的比较研究

目的对穴贴定喘膏体外透皮试验用皮肤做比较研究,选择适宜亲脂性成分的实验皮肤。方法应用TP-5型智能透皮扩散仪,以穴贴定喘膏中木兰脂素为考察指标,采用HPLC法测定,比较了不同种皮肤对穴贴定喘膏经皮渗透参数的影响。结果累积渗透量:硝酸纤维素>裸鼠>小鼠>壳聚糖>大鼠>兔>乳猪>蛇蜕;时滞:蛇蜕>壳聚糖>硝酸纤维素>乳猪>兔>大鼠>小鼠>裸鼠。结论硝酸纤维素膜可作为适宜亲脂性成分木兰脂素体外经皮渗透实验的理想皮肤模型。

Comparison of skin for percutaneous permeability of Xuetie Dingchuan Plaster in vitro

Objective To study the comparison of the skins for percutaneous permeability of Xuetie Dingchuan Plaster in vitro and make a regulation of pre-disposal method which evaluates percutanous permeability preliminarily for the excised skin in vitro. Methods Applying TP-5 automatic diffusion apparatus to determine magnolin in Xuetie Dingchuan Plaster by HPLC, comparison of the parameters of percutaneous permeability in different skins, lag time and amount of Xuetie Dingchuan Plaster permeability. Results Cumulative permeation amount: pyroxylin > hairless mouse > mouse > chitosan > rat > rabbit > porket > snake slough; Lag time: snake slough > chitosan > pyroxylin > porket > rabbit > rat > mouse > hairless mouse. Conclusion The cellulose nitrate filter can be regarded as a good model for the study of percutanous permeability of lipophilic magnolin.