新疆急慢性布鲁菌病患者炎性细胞因子免疫水平研究

Objective To investigate the cytokines IL-4, IL-6, IL-10, IL-17, TNF-α, INF-γ, and inflammatory factors PCT, hs-CRP and related factors RF, ESR, ASO, and blood culture in patients with acute and chronic brucellosis in Xinjiang. Methods Blood culture, sputum red plate, and test tube agglutination test were used to diagnose patients with Brucellosis (case group) and normal controls (control group). Experimental tests were performed using multiple microsphere flow immunofluorescence and biochemical techniques. Various cytokine and inflammatory factor indicators were analyzed using Stata 15.0 software. Results IL-6, IL-4, IL-10, IL-17, TNF-α and IFN-γ in the case group and the control group, were 2.74 (0.97-6.25) vs 0.56 (0.53-0.64), 34.10 (13.05-60.8) vs 0.78 (0.41-2.52), 1.83 (1.15-2.75) vs 0.89 (0.77-1.13), 6.77 (2.48-13.74) vs 1.03 (0.68-1.20), 35.23 (5.79-74.98) vs 1.33 (0.80-2.17), 212.96(42.3-436.72) pg/mL vs 1.69(1.36-1.89) pg/mL, and these factors in the case group were significantly higher than in the control group (P<0.05). IL-6, IL-10, IL-17, TNF-α and IFN-γ in the acute and chronic groups were 65.32(36.27-68.60) vs 29.38(11.30-53.09), 2.38(1.51-3.34) vs 0.89(0.77-1.13), 11.98 (3.92-16.49) vs 5.27 (2.24-12.49), 50.88 (16.59-96.69) vs 28.24 (5.30-64.40), 319.00 (145.14-588.52) pg/mL vs 160.52 (36.5-418.54) pg/mL, respectively, PCT and hs-CRP were 0.08 (0.06-0.12) pg/mL vs 0.04 (0.03-0.06) pg/mL, 12.60 (5.0-30.8) mg/L vs 3.30 (1.2-16.2) mg/L, respectively, with statistically significant differences (P<0.05). IL-6, PCT, hs-CRP, RF, ESR and the positive rates of blood culture in the low antibody titer group and the high antibody titer group were 30.46(11.30-50.02) pg/mL vs 46.20 (17.72-79.69) pg/mL, 0.04(0.03-0.06) pg/mL vs 0.07(0.04-0.11) pg/mL, 3.03 (0.85-9.70) mg/L vs 13.65 (2.97-31.43) mg/L, 3.0 (0.8-9.7) IU/mL vs 13.6 (3.0-31.4) IU/mL, 15 (7-31) mm/h vs 27 (15-46) mm/h, 15.3% vs 33.3%, respectively, the differences were statistically significant (P<0.05). Univariate analysis showed that all 6 cytokines were risk factors for brucellosis, and multivariate Logistic regression analysis showed that IL-6 and INF-γ levels were independent risk factors for brucellosis. Conclusion IL-4, IL-6, IL-10, IL-17, TNF-α, IFN-γ, and PCT, hs-CRP and other indicators can not only accurately and timely reflect the incidence and clinical significance of Brucella infection. Staged immune status, and IL-6 and INF - γ expression levels have independent clinical diagnostic evaluation. Blood culture has important clinical diagnostic value in the acute phase or high titer group. © 2020 China Tropical Medicine. All rights reserved.

Inflammatory cytokine immunity in patients with acute and chronic brucellosis in Xinjiang

Objective To investigate the cytokines IL-4, IL-6, IL-10, IL-17, TNF-α, INF-γ, and inflammatory factors PCT, hs-CRP and related factors RF, ESR, ASO, and blood culture in patients with acute and chronic brucellosis in Xinjiang. Methods Blood culture, sputum red plate, and test tube agglutination test were used to diagnose patients with Brucellosis(case group) and normal controls (control group). Experimental tests were performed using multiple microsphere flow immunofluorescence and biochemical techniques. Various cytokine and inflammatory factor indicators were analyzed using Stata 15.0 software. Results IL-6, IL-4, IL-10, IL-17, TNF-α and IFN-γ in the case group and the control group, were 2.74 (0.97-6.25) vs 0.56 (0.53-0.64), 34.10 (13.05-60.8) vs 0.78 (0.41-2.52), 1.83 (1.15-2.75) vs 0.89 (0.77-1.13), 6.77 (2.48-13.74) vs 1.03 (0.68-1.20), 35.23 (5.79-74.98) vs 1.33 (0.80-2.17), 212.96(42.3-436.72) pg/mL vs 1.69(1.36-1.89) pg/mL, and these factors in the case group were significantly higher than in the control group (P<0.05). IL-6, IL-10, IL-17, TNF-α and IFN-γ in the acute and chronic groups were 65.32(36.27-68.60) vs 29.38(11.30-53.09), 2.38(1.51-3.34) vs 0.89(0.77-1.13), 11.98 (3.92-16.49) vs 5.27 (2.24-12.49), 50.88 (16.59-96.69) vs 28.24 (5.30-64.40), 319.00 (145.14-588.52) pg/mL vs 160.52 (36.5-418.54) pg/mL, respectively, PCT and hs-CRP were 0.08 (0.06-0.12) pg/mL vs 0.04 (0.03-0.06) pg/mL, 12.60 (5.0-30.8) mg/L vs 3.30 (1.2-16.2) mg/L, respectively, with statistically significant differences (P<0.05). IL-6, PCT, hs-CRP, RF, ESR and the positive rates of blood culture in the low antibody titer group and the high antibody titer group were 30.46(11.30-50.02) pg/mL vs 46.20 (17.72-79.69) pg/mL, 0.04(0.03-0.06) pg/mL vs 0.07(0.04-0.11) pg/mL, 3.03 (0.85-9.70) mg/L vs 13.65(2.97-31.43) mg/L, 3.0 (0.8-9.7) IU/mL vs 13.6 (3.0-31.4) IU/mL, 15 (7-31) mm/h vs 27 (15-46) mm/h, 15.3% vs 33.3%, respectively, the differences were statistically significant (P<0.05). Univariate analysis showed that all 6 cytokines were risk factors for brucellosis, and multivariate Logistic regression analysis showed that IL-6 and INF-γ levels were independent risk factors for brucellosis. Conclusion IL-4, IL-6, IL-10, IL-17, TNF-α, IFN-γ, and PCT, hs-CRP and other indicators can not only accurately and timely reflect the incidence and clinical significance of Brucella infection. Staged immune status, and IL-6 and INF-γ expression levels have independent clinical diagnostic evaluation. Blood culture has important clinical diagnostic value in the acute phase or high titer group.