有氧运动和膳食脂肪对ApoE基因缺陷小鼠动脉粥样硬化斑块形成过程中SR-B1基因及蛋白表达的影响

为研究有氧运动和低脂膳食抑制动脉粥样硬化斑块形成的可能机制，本研究探讨了１０周有氧运动和低脂膳食对ＡｐｏＥ基因缺陷小鼠主动脉动脉粥样硬化斑块面积及肝脏ＳＲ－Ｂ１ｍＲＮＡ和蛋白水平的影响。结果表明有氧运动和／或低脂膳食增加ＡｐｏＥ基因缺陷小鼠肝脏ＳＲ－ＢＩ基因及蛋白的表达水平。肝脏ＳＲ－Ｂ１ｍＲＮＡ水平与动脉粥样硬化斑块间存在负相关关系的趋势（相关系数ｒ值为－０．８１４，显著性检验Ｐ值为０．０６２），而且ＳＲ－Ｂ１蛋白水平与动脉粥样硬化斑块间也存在负相关关系（相关系数ｒ值为－０．８７１，显著性检验的Ｐ值为０．０１４）。结果提示在动脉粥样硬化斑块形成过程中，有氧运动和低脂膳食引起的ＳＲ－Ｂ１基因及蛋白表达水平的增加可能是二者具有抗动脉粥样硬化斑块发生作用的潜在机制。

Effect of Aerobic Exercise and Dietary Fat on SR-B1 Gene and Protein Expression during the Formation of Atherosclerotic Plaque in ApoE-Deficient Mice

To investigate inhibition of the antiatherosclerotic plaque formation mechanism of aerobic exercise and low fat diet, we studied the effects of 10-week aerobic exercise and low fat diet on the atherosclerotic plaque area in the aorta and liver SR-B1 mRNA and SR-B1 protein level in ApoE-Deficient mice. The results show that aerobic exercise and /or low fat diet increase SR-B1 mRNA and SR-B1 protein expression in ApoE-Deficient mice liver. SR-B1 mRNA trends to reversely correlated with atherosclerotic plaque area in ApoE-Deficient mice (r=-0.814 and P= 0.062), while SR R 1 protein level are significantly reversely correlated with the atherosclerotic plaque area in ApoE-Deficient mice (r=-0.871 and P=O.014). The results imply that higher SR- B1 gene and protein level caused by aerobic exercise and low fat diet may be one of the potential antiatherosclerotic mechanisms.