| 兔软骨细胞培养方法研究 |
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| 引用本文： | 魏西秦,曹峻岭,熊咏民. 兔软骨细胞培养方法研究[J]. 中国地方病学杂志, 1987, 0(2) |
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| 作者姓名： | 魏西秦 曹峻岭 熊咏民 |
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| 作者单位： | 西安医科大学病理解剖教研室(魏西秦，曹峻岭)，西安医科大学病理解剖教研室(熊咏民) |
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| 摘 要： | Manning 和 Bonner1967年首先把胰蛋白酶和细菌胶原酶结合使用消化关节软骨基质获得大量纯度高的软骨细胞,此后,软骨细胞培养研究工作得到很大发展并取得丰硕成果。现这一方法经改进后在国外已被广泛使用,在我国尚未见有关这方面报导。本文以体外单层兔软骨细胞培养为例,简要介绍用透明质酸酶、胰蛋白酶和胶原酶消化分离兔关节软骨细胞的实验材料准备,操作方法步骤,并对实验结果作了简要讨论。希望通过这一方法介绍,对从事软骨疾病研究、大骨节病病因研究人员有所助益。
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| 关 键 词： | 细胞培养 软骨细胞 软骨细胞单层培养 |
A METHOD OF MONOLAYER CULTURE OF RABBIT CHONDROCYTES |
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| Wei Xi-qin. A METHOD OF MONOLAYER CULTURE OF RABBIT CHONDROCYTES[J]. Chinese Jouranl of Endemiology, 1987, 0(2) |
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| Authors: | Wei Xi-qin |
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| Abstract: | An old teaching that chondrocytes of mature joint cartilage are unableto replicate themselves,was disproved by cell culture in 1967 by Manning andBonner.This awaited the recognition that viable chondrocytes could bereleased from even tough cartilages by bacterial collagenase.Since then,thetremendous achievementes in the field of chondrocytes culture have beenmade.Isolation of the chondrocyte depends upon enzymatic removal of thematrix.This paper deals with the method of Monolayer Culture of rabbitchondrocytes which were isolated by testicular hyaluronidase,trypsin andclostridial collagenase. |
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| Keywords: | Cell culture Chondrocytes Monolayer culture of chondrocytes |
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