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| 完全弗氏佐剂诱导脾脏T淋巴细胞凋亡预防非肥胖性糖尿病鼠1型糖尿病 | |
| 引用本文： | 王晓希 牛晓红 等. 完全弗氏佐剂诱导脾脏T淋巴细胞凋亡预防非肥胖性糖尿病鼠1型糖尿病[J]. 湖南医科大学学报, 2002, 27(2): 105-107 |
| 作者姓名： | 王晓希 牛晓红 等 |
| 摘 要： | 为探讨脾脏T淋巴细胞凋亡在完全弗氏佐剂（CFA）预防非肥胖性糖尿病（NOD）鼠糖尿病中的作用，42只NOD雌鼠随机分成CFA组（21只）和磷酸盐缓冲盐水（PBS）组（21只），3周龄时于鼠后脚板注射CFA50μl/只和PBS50μl／只。按不同的观察时间分6周、12周和30周3个观察组。采用TUNEL末端标记法和免疫组织化学方法观察脾脏T淋巴细胞凋亡情况。结果显示：CFA诱导脾脏成熟的CD4^ T淋巴细胞凋亡，但不能诱导成熟的CD8^ T淋巴细胞凋亡；CFA能预防NOD鼠糖尿病的发生。提示CFA可通过诱导脾脏T淋巴细胞凋亡，以减少外周的CD4^ T淋巴细胞，达到预防NOD鼠1型糖尿病。 |
| 关 键 词： | 完全弗氏佐剂 脾脏 T淋巴细胞 预防 非肥胖性糖尿病 1型糖尿病 细胞凋亡 动物疾病模型 |
Complete Freund''s adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice] |
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| Xiao-xi Wang,Xiao-hong Niu,Zhi-guang Zhou. Complete Freund''s adjuvant-induced splenic T cell apoptosis in the prevention of diabetes in non-obese diabetic mice][J]. Bulletin of Hunan Medical University, 2002, 27(2): 105-107 | |
| Authors: | Xiao-xi Wang Xiao-hong Niu Zhi-guang Zhou |
| Affiliation: | Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China. |
| Abstract: | OBJECTIVE: To study the apoptosis of T lymphocytes in the spleen and its role in complete Freund's adjuvant (CFA) preventing diabetes in non-obese diabetic (NOD) mice. METHODS: Forty-two NOD mice were divided into two groups randomly: CFA-treated NOD female mice (6w, 12w and 30w) and PBS-treated NOD female mice (6w, 12w and 30w). The apoptosis of splenic T lymphocytes was observed with the TDT-mediated fluorescein-dUTP nick-end labelling (TUNEL) technique and avidin-biotin complex method. RESULTS: CFA induced the apoptosis of CD4+ T lymphocytes in the mouse spleen and could prevent the development of Type 1 diabetes (T1DM) in NOD female mice. CONCLUSION: CFA preventing Type 1 diabetes in NOD mice may be related to the apoptosis of T lymphocytes in the spleen, which downregulates mature CD4+ T lymphocytes in the periphery. |
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