Production and characterisation of anti-theophylline monoclonal antibodies suitable for immunoassay |
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| Authors: | N D Danilova R G Vasilov |
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| Affiliation: | Department of Medical Diagnostics, Institute of Biotechnology, Moscow, U.S.S.R. |
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| Abstract: | ![]()
Spleen cells from BALB/c mice immunised with KLH-theophylline conjugate were fused with a mouse myeloma cell line P3-X63-Ag8.653, and antibody-producing hybrids were identified by enzyme immunoassay. Three cell clones were obtained, each capable of producing a unique monoclonal antibody to theophylline. Using these monoclonal antibodies, an immunoassay system for theophylline was developed. |
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