| 硼替佐米和柔红霉素联合应用对多发性骨髓瘤细胞株KM3的作用 |
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| 引用本文： | 欧阳桂芳,林茂芳. 硼替佐米和柔红霉素联合应用对多发性骨髓瘤细胞株KM3的作用[J]. 中国实验血液学杂志, 2009, 17(6): 1468-1471 |
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| 作者姓名： | 欧阳桂芳 林茂芳 |
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| 作者单位： | 1. 宁波大学医学院附属宁波市第一医院血液科,浙江宁波,315010
2. 浙江大学附属第一医院血液科,浙江杭州,310003 |
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| 摘 要： | 为了研究硼替佐米（bortezomib，Bor）单用及与柔红霉素（daunorubicin，DNR）联合应用对多发性骨髓瘤（multiple mydoma，MM）细胞株KM3细胞的抑制作用，采用MTT法检测Bor单用及与DNR联合应用对KM3细胞的抑制作用，求出其IC50。结果表明：Bor、DNR对KM3细胞生长抑制作用呈浓度依赖性，IC50分别为0．27μmoL／L，0．16μmol／L；Bor联合DNR对KM3细胞生长抑制作用明显增强（P〈0．05）。结论：在体外，Bor联合DNR对KM3细胞生长抑制有协同作用。
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| 关 键 词： | 硼替佐米 柔红霉素 多发性骨髓瘤细胞 KM3细胞株 |
Combined Effects of Bortezomib and Daunorubicin on Multiple Myeloma Cell KM3 In Vitro |
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| OUYANG Gui-Fang,LIN Mao-Fang. Combined Effects of Bortezomib and Daunorubicin on Multiple Myeloma Cell KM3 In Vitro[J]. Journal of experimental hematology, 2009, 17(6): 1468-1471 |
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| Authors: | OUYANG Gui-Fang LIN Mao-Fang |
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| Affiliation: | ( Department of Hematology, The First Hospital of Ningbo , Ningbo University Medical College, Ningbo 315010 Zhejiang Province, China; 1 Department of Hematology, The First Hospital, Zhejiang University Medical College, Hangzhou 310003, Zhejiang Province, China) |
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| Abstract: | The aim of this study was to investigate the combined effects of bortezomib (Bor)and daunorubicin fDNR)or each drug alone on proliferation of human mumple myeloma cell line KM3.KM3 cells Were cultured with different concentrations of Bor and DNR.Bor or DNR alone for different times.The cell proliferation was analyzed by MTr assay,and the concentration of 50% growth inhibition(IC50) was calculated.The results indicated that both of Bor and DNR inhibited KM3 cell proliferation in dose dependent nlanner.The IC50 of both drugs Were 0.27 μmol/L and 0.16 μmoi/L respectively.The inhibiting rate of Bor plus DNR on KM3 cells was much higher than that of Bor(P<0.05).It is concluded that the Bor has synergistic inhibitory effect with DNR on the growth of KM3 cell in vitro. |
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| Keywords: | bortezomib:daunombicin:multipie myeloma cell:KMB cell line |
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