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| 苦参碱对人胃癌细胞NCI-N87体外的增殖抑制作用 | |
| 引用本文： | 王中林,蒋平,于永洋. 苦参碱对人胃癌细胞NCI-N87体外的增殖抑制作用[J]. 中国中医急症, 2007, 16(6): 706-708 |
| 作者姓名： | 王中林 蒋平 于永洋 |
| 作者单位： | 温州医学院附属第二医院,温州,325027 |
| 基金项目： | 浙江省温州市科技局资助项目;浙江省卫生厅资助项目 |
| 摘 要： | 目的探讨不同浓度苦参碱对体外培养的人胃癌细胞NCI-N87的增殖抑制作用。方法NCI-N87细胞经不同浓度苦参碱处理后,采用CCK-8法检测药物对肿瘤细胞的抑制作用,观察细胞形态学改变,检测细胞DNA分布。结果苦参碱浓度>3.0mg/ml,细胞毒作用较大;浓度在0.5～2.0mg/ml时,对肿瘤细胞的生长有抑制作用,且呈时间剂量依赖性;浓度在0.1mg/ml时,对肿瘤细胞生长几乎无抑制作用。苦参碱作用后可增加G1期细胞所占的百分比,阻止细胞进入S期。结论苦参碱对人胃癌细胞NCI-N87增殖有抑制作用,并能阻抑细胞周期的进程。 |
| 关 键 词： | 苦参碱 胃癌 细胞增殖 |
| 文章编号： | 1004-745X（2007）06-0706-03 |
| 收稿时间： | 2006-12-12 |
| 修稿时间： | 2006-12-12 |
The Inhibitory Effect of Matrine on the Proliferation of Human Gastric Cancer Cell Line NCI-N87 in Vitro |
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| WANG Zhong-lin,JIANG Ping,YU Yong-yang. The Inhibitory Effect of Matrine on the Proliferation of Human Gastric Cancer Cell Line NCI-N87 in Vitro[J]. Journal of Emergency in Traditional Chinese Medicine, 2007, 16(6): 706-708 | |
| Authors: | WANG Zhong-lin JIANG Ping YU Yong-yang |
| Affiliation: | The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China |
| Abstract: | Objective:To investigate the inhibition of different concentrations of matrine on Proliferation of human gastric cancer cell line NCI-N87 cultured in vitro.Methods:NCI-N87 cells were treated by matrine in different concentrations and variablc durations.Cell growth and proliferation were observed by CCK-8 assay.Morphological changs of cell were observed,and cell-DNA distribution was assayed.Results:When the drug concentration was higher than 3.0mg/ml,the major effect was cell mecrosis.Matrine in the concentrations from 0.5mg/ml to 2.0mg/ml could inhibit proliferation of NCI-N87 cells as reveled by CCK-8 assay.Increase in the percentage of Gl phase cell was observed by flow cytometry.Conclusion:Matrine can inhibit the proliferation of human gastric cancer cell line NCI-N87 efficiently by affecting the cycle of cells. |
| Keywords: | Matrine gastric cancer proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |