一氧化氮及血管活性肠肽在实验性反流性食管炎发病机制中的作用

目的：探讨一氧化氮（NO）及血管活性肠肽（VIP）在实验性反流性食管炎（RE）发病机制中的应用,方法：18只SD大鼠随机分为A、B、C3组,A、B两组手术造成RE,C组采用伪开腹术。2－3月时免疫组化法及彩色图像分析仪对3组大鼠食管组织中的NO及VIP含量进行定性。结果：A、B两组大鼠均发生RE,A组引起有溃疡的RE的比例比B组高。A、B两组大鼠食管壁各层组织中NO能和VIP能阳性神经和其阳性产物显著多于C组,A、B两组之间无明显差异。结论：单纯十二指肠液反流可引起RE,其致炎作用可能较混合反流要强,实验性RE大鼠食管组织中NO组、VIP能阳性神经显著增加,提示NO能和VIP能阳性神经在RE的发生机制中起了 重要的作用。

Effects of nitric oxide and vasoactive intestinal polypeptide in experimental reflux esophagitis

Objective: To study the effects of nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) in the pathogenesis of experimental reflux esophagitis (RE). Methods: Eighteen SD rats were randomly divided into A,B,C groups. A,B groups were induced to RE by operation, C group had false abdominal operation. All rats were killed 2 3 months later and the NO,VIP neurons were investigated qualitatively and quantitatively by immunohistochemical method and color image analyzer. Results: RE occurred in all rats in groups A and B. The rate of ulcer RE in group A was more than that in group B. In group A,B, the NO and VIP positive neurons and positive products were greatly increased with respect to group C,but no significant difference was observed between group A and B. Conclusion: RE can be induced by sole duodenal juice reflux.Duodenal juice may be more inflaming than mixing reflux juice.The findings that the NO and VIP positive neurons are significant increased in experimental RE groups indicate that NO and VIP neurons play important roles in the pathogenesis of RE. [