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神经节苷脂对脑缺氧缺血后新生大鼠海马CA1区 N-甲基-D-天门冬氨酸受体表达的影响
引用本文:曹艳华,王玲,惠延平,王宝西,曹玉红. 神经节苷脂对脑缺氧缺血后新生大鼠海马CA1区 N-甲基-D-天门冬氨酸受体表达的影响[J]. 实用儿科临床杂志, 2002, 17(6): 614-616
作者姓名:曹艳华  王玲  惠延平  王宝西  曹玉红
作者单位:710038,西安,第四军医大学唐都医院儿科
摘    要:目的 研究新生大鼠缺氧缺血性脑损伤(HIBD)后海马区N-甲基-D-天门冬氨酸受体(NMDAR)及NMDARmRNA表达的变化和神经节苷脂(GM1)对其的影响。方法 建立HIBD模型。用免疫组化及原位杂交方法检测缺氧缺血(HI)和GM1干预后不同时间点NMDA受体I型亚单位(NR1)阳性细胞及NR1mRNA阳性细胞的表达。结果 HI后新生大鼠海马CA1区NR1及NR1mRNA表达增强,GM1可使其受体表达下调(P<0.05)。结论 GM1可使新生大鼠海马CA1区NR1及NR1mRNA表达下调,对新生大鼠缺氧缺血性脑损伤具有保护作用。

关 键 词:神经节苷脂 脑缺氧缺血 新生大鼠 海马 CA1区 N-甲基-D-天门冬氨酸受体
文章编号:1003-515X(2002)06-0614-03
修稿时间:2002-06-30

Effect of gangliosides on N-methy-D-aspartate receptor expression in hippocampal CA1 in neonatal rat models with cerebral hy-poxic-ischemia
CAO Yanhua,WANG Ling,HUI Yanping,et al.. Effect of gangliosides on N-methy-D-aspartate receptor expression in hippocampal CA1 in neonatal rat models with cerebral hy-poxic-ischemia[J]. Journal of Applied Clinical Pediatrics, 2002, 17(6): 614-616
Authors:CAO Yanhua  WANG Ling  HUI Yanping  et al.
Affiliation:CAO Yanhua,WANG Ling,HUI Yanping,et al.Department of Pediatrics,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,P.R.China
Abstract:Objective To study the expression of N-methy-D-aspartate receptor(NMDAR) and NMDAR mRNA in hip-pocampal CA1 and the effect of monosialotetrahexosylganglioside(GM1) on the expression after hypoxic-ischemic brain damage(HIBD) in neonatal rats. Methods An animal model of neonatal hypoxic-ischemic brain damage was established. The expression of NR1 positive cells was detected with NR1 immunohistochemisry and NR1 mRNA in situs hybridization. Results The expression of positive cells was observed in hippocampal CA1 in neonatal rats NR1 positive cells and NR1 mRNA positive cells were increased in hippocampal CA1 area after hypoxic-ischemia (HI). The expression of NR1 and NR1 mRNA was down-regulated after GM1 administration in GM1 group compared to that of HI group. Conclusion GM1, as antagonist of the NMDAR, plays a role in brain protection against hypoxic-ischemia.
Keywords:gangliosides  N-methy-D-aspartate receptor  hypoxic-ischemia  neonatal rat
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