急性冠状动脉综合征患者基质金属蛋白酶9基因多态性

目的 探讨基质金属蛋白酶9基因C1562T多态性与中国南方汉族人群冠心病的关系.方法 对经冠状动脉造影证实的急性冠状动脉综合征患者150例(急性冠状动脉综合征组)、稳定型心绞痛患者110例(稳定型心绞痛组)和同期冠状动脉造影阴性、排除冠心病诊断的患者70例(对照组)进行研究,采用酶联免疫吸附试验测定血浆基质金属蛋白酶9水平,采用聚合酶链反应-限制片长多态性技术分析基质金属蛋白酶9基因中C1562T基因多态性,比较各组的基因型和等位基因频率.结果 急性冠状动脉综合征组血浆基质金属蛋白酶9水平明显高于稳定型心绞痛组(P＜0.05)和对照组(P＜0.01),而稳定型心绞痛组与对照组比较,差异无统计学意义(P＞0.05).急性冠状动脉综合征组基质金属蛋白酶9基因CT、CT TT基因型频率以及T等位基因频率均高于对照组和稳定型心绞痛组(P＜0.05或0.01),稳定型心绞痛组与对照组各基因型和等位基因频率分布差异无统计学意义(P＞0.05).C1562T位点CT/TT基因型患者血浆基质金属蛋白酶9水平显著高于CC基因型患者(P＜0.01).结论 基质金属蛋白酶9基因C1562T多态性可能与中国南方汉族人群急性冠状动脉综合征有关,1562T等位基因是动脉粥样硬化斑块不稳定性的易感基因.

The Relationship Between Matrix Metalloproteinase-9 Polymorphism (C1562T) and Acute Coronary Syndrome

Aim To investigate the association between matrix metalloproteinase-9 (MMP-9) polymorphism (C1562T) and coronary heart disease (CHD) in Han population of South China. Methods The study included 150 patients with acute coronary syndrome (ACS group), 110 patients with stable angina pectoris (SAP group) and 70 controls (control group) , all of which were confirmed by angiocardiography. Plasma levels of MMP-9 were measured by enzyme-linked immunosorbent assay (ELISA). Promoter C1562T gene polymorphism of MMP-9 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype and allele frequency were compared among groups. Results Plasma MMP-9 level in ACS group were significantly higher than those of SAP group (P<0.05) and control group (P<0.01), however there was no difference between SAP group and control group (P>0.05). The frequency of CT and CT TT genotype and T allele were higher in ACS group compared with those in control group and SAP group (P<0.05 or 0.01), but the frequencies of C/T genotypes and alleles were not statistically different between SAP group and control group (P>0.05). Plasma MMP-9 level of CT/TT genetype was higher than that of CC genetype (P<0.01). Conclusion The genetic polymorphism in MMP-9 promoter (C1562T) is associated with ACS in the Han population of South China, and T allele is a susceptible gene of unstability of atherosclerosis plaque.